RNA microarray for estimating relative abundance of 16S rRNA in microbial communities

Tatsuhiko Hoshino; Kazuhiro Furukawa; Satoshi Tsuneda; Yuhei Inamori

doi:10.1016/j.mimet.2007.01.007

RNA microarray for estimating relative abundance of 16S rRNA in microbial communities

Tatsuhiko Hoshino^*, Kazuhiro Furukawa, Satoshi Tsuneda, Yuhei Inamori

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

We developed an RNA microarray protocol in which total RNA from a microbial community was attached to a slide glass, and rRNA was detected by fluorescently labeled oligonucleotide probes. The RNA microarray requires only 4 h for hybridization and enables double staining and estimating relative abundance of rRNA.

Original language	English
Pages (from-to)	406-410
Number of pages	5
Journal	Journal of Microbiological Methods
Volume	69
Issue number	2
DOIs	https://doi.org/10.1016/j.mimet.2007.01.007
Publication status	Published - 2007 May

Keywords

Hybridization
RNA microarray
Relative abundance

ASJC Scopus subject areas

Microbiology
Molecular Biology
Microbiology (medical)

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10.1016/j.mimet.2007.01.007

Cite this

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title = "RNA microarray for estimating relative abundance of 16S rRNA in microbial communities",

abstract = "We developed an RNA microarray protocol in which total RNA from a microbial community was attached to a slide glass, and rRNA was detected by fluorescently labeled oligonucleotide probes. The RNA microarray requires only 4 h for hybridization and enables double staining and estimating relative abundance of rRNA.",

keywords = "Hybridization, RNA microarray, Relative abundance",

author = "Tatsuhiko Hoshino and Kazuhiro Furukawa and Satoshi Tsuneda and Yuhei Inamori",

year = "2007",

month = may,

doi = "10.1016/j.mimet.2007.01.007",

language = "English",

volume = "69",

pages = "406--410",

journal = "Journal of Microbiological Methods",

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TY - JOUR

T1 - RNA microarray for estimating relative abundance of 16S rRNA in microbial communities

AU - Hoshino, Tatsuhiko

AU - Furukawa, Kazuhiro

AU - Tsuneda, Satoshi

AU - Inamori, Yuhei

PY - 2007/5

Y1 - 2007/5

N2 - We developed an RNA microarray protocol in which total RNA from a microbial community was attached to a slide glass, and rRNA was detected by fluorescently labeled oligonucleotide probes. The RNA microarray requires only 4 h for hybridization and enables double staining and estimating relative abundance of rRNA.

AB - We developed an RNA microarray protocol in which total RNA from a microbial community was attached to a slide glass, and rRNA was detected by fluorescently labeled oligonucleotide probes. The RNA microarray requires only 4 h for hybridization and enables double staining and estimating relative abundance of rRNA.

KW - Hybridization

KW - RNA microarray

KW - Relative abundance

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M3 - Article

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AN - SCOPUS:34047256256

SN - 0167-7012

VL - 69

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EP - 410

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 2

ER -

RNA microarray for estimating relative abundance of 16S rRNA in microbial communities

Abstract

Keywords

ASJC Scopus subject areas

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