Rotational dynamics of immunoglobulins with fluorescent haptens on a membrane surface

Hiroshi Osada; Mamoru Nakanishi; Masamichi Tsuboi; Kazuhiko Kinosita; Akira Ikegami

doi:10.1016/0005-2736(84)90097-X

Rotational dynamics of immunoglobulins with fluorescent haptens on a membrane surface

Hiroshi Osada, Mamoru Nakanishi^*, Masamichi Tsuboi, Kazuhiko Kinosita, Akira Ikegami

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The rotational dynamics of rabbit immunoglobulin G with fluorescent lipid haptens on a membrane surface has been studied by nanosecond fluorescence emission anisotropic spectroscopy. It has been found that the rotational angles of the antibody are very restricted on the membrane, but that the rotation rate itself is not appreciably lower than that in solution, and is independent of the membrane fluidity.

Original language	English
Pages (from-to)	321-324
Number of pages	4
Journal	BBA - Biomembranes
Volume	773
Issue number	2
DOIs	https://doi.org/10.1016/0005-2736(84)90097-X
Publication status	Published - 1984 Jun 27
Externally published	Yes

Keywords

(Liposome)
Fluorescence anisotropy
Immunoglobulin
Lipid hapten
Membrane fluidity
Rotational dynamics

ASJC Scopus subject areas

Biochemistry
Biophysics
Cell Biology
Medicine(all)

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10.1016/0005-2736(84)90097-X

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abstract = "The rotational dynamics of rabbit immunoglobulin G with fluorescent lipid haptens on a membrane surface has been studied by nanosecond fluorescence emission anisotropic spectroscopy. It has been found that the rotational angles of the antibody are very restricted on the membrane, but that the rotation rate itself is not appreciably lower than that in solution, and is independent of the membrane fluidity.",

keywords = "(Liposome), Fluorescence anisotropy, Immunoglobulin, Lipid hapten, Membrane fluidity, Rotational dynamics",

author = "Hiroshi Osada and Mamoru Nakanishi and Masamichi Tsuboi and Kazuhiko Kinosita and Akira Ikegami",

year = "1984",

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doi = "10.1016/0005-2736(84)90097-X",

language = "English",

volume = "773",

pages = "321--324",

journal = "Biochimica et Biophysica Acta - Biomembranes",

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T1 - Rotational dynamics of immunoglobulins with fluorescent haptens on a membrane surface

AU - Osada, Hiroshi

AU - Nakanishi, Mamoru

AU - Tsuboi, Masamichi

AU - Kinosita, Kazuhiko

AU - Ikegami, Akira

PY - 1984/6/27

Y1 - 1984/6/27

N2 - The rotational dynamics of rabbit immunoglobulin G with fluorescent lipid haptens on a membrane surface has been studied by nanosecond fluorescence emission anisotropic spectroscopy. It has been found that the rotational angles of the antibody are very restricted on the membrane, but that the rotation rate itself is not appreciably lower than that in solution, and is independent of the membrane fluidity.

AB - The rotational dynamics of rabbit immunoglobulin G with fluorescent lipid haptens on a membrane surface has been studied by nanosecond fluorescence emission anisotropic spectroscopy. It has been found that the rotational angles of the antibody are very restricted on the membrane, but that the rotation rate itself is not appreciably lower than that in solution, and is independent of the membrane fluidity.

KW - (Liposome)

KW - Fluorescence anisotropy

KW - Immunoglobulin

KW - Lipid hapten

KW - Membrane fluidity

KW - Rotational dynamics

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EP - 324

JO - Biochimica et Biophysica Acta - Biomembranes

JF - Biochimica et Biophysica Acta - Biomembranes

IS - 2

ER -

Rotational dynamics of immunoglobulins with fluorescent haptens on a membrane surface

Abstract

Keywords

ASJC Scopus subject areas

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