TY - JOUR
T1 - Saccharomonospora colocasiae sp. Nov., an actinomycete isolated from the rhizosphere of Colocasia esculenta
AU - Wattanasuepsin, Watsapon
AU - Intra, Bungonsiri
AU - Take, Akira
AU - Inahashi, Yuki
AU - Euanorasetr, Jirayut
AU - Ōmura, Satoshi
AU - Matsumoto, Atsuko
AU - Panbangred, Watanalai
PY - 2017/11/1
Y1 - 2017/11/1
N2 - A non-Streptomyces actinomycete, designated as strain S265T, was isolated from rhizosphere collected under an elephant ear plant (Colocasia esculenta) in Bangkok, Thailand. The taxonomic position of this strain was determined by a polyphasic approach. Strain S265T formed single globose spores on long, branching, aerial hyphae. It produced abundant aerial mycelium with green colour. The cell wall contained meso-diaminopimelic acid, and diagnostic whole-cell sugars were arabinose and galactose. Phosphatidylethanolamine and diphosphatidylglycerol were detected predominantly as polar lipids, whereas mycolic acids were not found. The major menaquinone was MK-9(H4), and principal cellular fatty acids were C15: 1 B, iso-C16: 1 H, anteiso-C15: 0 and C15: 0 2-OH. The DNA G+C content was 69 mol%. According to phylogenetic analysis, strain S265T was clustered with Saccharomonospora glauca K62T (98.1%) and Saccharomonospora viridis DSM 43017T (97.1%) despite its 16S rRNA gene sequence showing the highest similarity value to that of Saccharomonospora azurea NA-128T (98.6%). DNA–DNA relatedness values between strain S265T and the closely related strains were in the range of 7–50%, thus strengthening the evidence derived from the polyphasic study that strain S265T represents a novel species within the genus Saccharomonospora, for which the name Saccharomonospora colocasiae sp. nov. is proposed. The type strain is S265T (=TBRC 7235T=NBRC 112945T).
AB - A non-Streptomyces actinomycete, designated as strain S265T, was isolated from rhizosphere collected under an elephant ear plant (Colocasia esculenta) in Bangkok, Thailand. The taxonomic position of this strain was determined by a polyphasic approach. Strain S265T formed single globose spores on long, branching, aerial hyphae. It produced abundant aerial mycelium with green colour. The cell wall contained meso-diaminopimelic acid, and diagnostic whole-cell sugars were arabinose and galactose. Phosphatidylethanolamine and diphosphatidylglycerol were detected predominantly as polar lipids, whereas mycolic acids were not found. The major menaquinone was MK-9(H4), and principal cellular fatty acids were C15: 1 B, iso-C16: 1 H, anteiso-C15: 0 and C15: 0 2-OH. The DNA G+C content was 69 mol%. According to phylogenetic analysis, strain S265T was clustered with Saccharomonospora glauca K62T (98.1%) and Saccharomonospora viridis DSM 43017T (97.1%) despite its 16S rRNA gene sequence showing the highest similarity value to that of Saccharomonospora azurea NA-128T (98.6%). DNA–DNA relatedness values between strain S265T and the closely related strains were in the range of 7–50%, thus strengthening the evidence derived from the polyphasic study that strain S265T represents a novel species within the genus Saccharomonospora, for which the name Saccharomonospora colocasiae sp. nov. is proposed. The type strain is S265T (=TBRC 7235T=NBRC 112945T).
KW - Rhizosphere
KW - Saccharomonospora
UR - http://www.scopus.com/inward/record.url?scp=85034215022&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85034215022&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.002336
DO - 10.1099/ijsem.0.002336
M3 - Article
C2 - 28945543
AN - SCOPUS:85034215022
SN - 1466-5026
VL - 67
SP - 4572
EP - 4577
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 11
ER -