Search for immunosuppressive substances produced in the culture of enteric bacteria isolated from marine fish

Hitoshi Ohmori; Masayuki Raitoh; Mikiya Harano; Toshiyuki Oda; Masaki Hikida; Ryosuke Sugihara; Kazunaga Yazawa

doi:10.1016/S0922-338X(97)80365-0

Search for immunosuppressive substances produced in the culture of enteric bacteria isolated from marine fish

Hitoshi Ohmori^*, Masayuki Raitoh, Mikiya Harano, Toshiyuki Oda, Masaki Hikida, Ryosuke Sugihara, Kazunaga Yazawa

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Culture supernatants of enteric bacteria isolated from the gastrointestinal tracts of two marine fishes were screened for the production of immunosuppressive substances. At least 50% of the 60 bacterial isolates tested were found to produce immunosuppressants that significantly inhibited the induction of cytotoxic T cells (CTL) in vitro. The strains that secreted the highest suppressive activity was identified as Aeromonas caviae. The immunosuppressive substance purified from the A. caviae culture supernatant was a polysaccharide with a molecular weight of about 70 kDa that contained mannose : glucose (4:1). This polysaccharide did not show nonspecific cytotoxicity, and was found to inhibit the proliferation and differentiation of CD8-positive CTL.

Original language	English
Pages (from-to)	117-119
Number of pages	3
Journal	Journal of Fermentation and Bioengineering
Volume	85
Issue number	1
DOIs	https://doi.org/10.1016/S0922-338X(97)80365-0
Publication status	Published - 1998
Externally published	Yes

Keywords

Cytotoxic T cells
Fish enteric bacteria
Immune suppression
Polysaccharides

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0922-338X(97)80365-0

Cite this

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title = "Search for immunosuppressive substances produced in the culture of enteric bacteria isolated from marine fish",

abstract = "Culture supernatants of enteric bacteria isolated from the gastrointestinal tracts of two marine fishes were screened for the production of immunosuppressive substances. At least 50% of the 60 bacterial isolates tested were found to produce immunosuppressants that significantly inhibited the induction of cytotoxic T cells (CTL) in vitro. The strains that secreted the highest suppressive activity was identified as Aeromonas caviae. The immunosuppressive substance purified from the A. caviae culture supernatant was a polysaccharide with a molecular weight of about 70 kDa that contained mannose : glucose (4:1). This polysaccharide did not show nonspecific cytotoxicity, and was found to inhibit the proliferation and differentiation of CD8-positive CTL.",

keywords = "Cytotoxic T cells, Fish enteric bacteria, Immune suppression, Polysaccharides",

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year = "1998",

doi = "10.1016/S0922-338X(97)80365-0",

language = "English",

volume = "85",

pages = "117--119",

journal = "Journal of Fermentation and Bioengineering",

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TY - JOUR

T1 - Search for immunosuppressive substances produced in the culture of enteric bacteria isolated from marine fish

AU - Ohmori, Hitoshi

AU - Raitoh, Masayuki

AU - Harano, Mikiya

AU - Oda, Toshiyuki

AU - Hikida, Masaki

AU - Sugihara, Ryosuke

AU - Yazawa, Kazunaga

PY - 1998

Y1 - 1998

N2 - Culture supernatants of enteric bacteria isolated from the gastrointestinal tracts of two marine fishes were screened for the production of immunosuppressive substances. At least 50% of the 60 bacterial isolates tested were found to produce immunosuppressants that significantly inhibited the induction of cytotoxic T cells (CTL) in vitro. The strains that secreted the highest suppressive activity was identified as Aeromonas caviae. The immunosuppressive substance purified from the A. caviae culture supernatant was a polysaccharide with a molecular weight of about 70 kDa that contained mannose : glucose (4:1). This polysaccharide did not show nonspecific cytotoxicity, and was found to inhibit the proliferation and differentiation of CD8-positive CTL.

AB - Culture supernatants of enteric bacteria isolated from the gastrointestinal tracts of two marine fishes were screened for the production of immunosuppressive substances. At least 50% of the 60 bacterial isolates tested were found to produce immunosuppressants that significantly inhibited the induction of cytotoxic T cells (CTL) in vitro. The strains that secreted the highest suppressive activity was identified as Aeromonas caviae. The immunosuppressive substance purified from the A. caviae culture supernatant was a polysaccharide with a molecular weight of about 70 kDa that contained mannose : glucose (4:1). This polysaccharide did not show nonspecific cytotoxicity, and was found to inhibit the proliferation and differentiation of CD8-positive CTL.

KW - Cytotoxic T cells

KW - Fish enteric bacteria

KW - Immune suppression

KW - Polysaccharides

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DO - 10.1016/S0922-338X(97)80365-0

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Search for immunosuppressive substances produced in the culture of enteric bacteria isolated from marine fish

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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