TY - JOUR
T1 - Selective detection of Escherichia coli by imaging of the light intensity transmitted through an optical disk
AU - Shiramizu, Hideyuki
AU - Kuroda, Chiaki
AU - Ohki, Yoshimichi
AU - Shima, Takayuki
AU - Wang, Xiaomin
AU - Fujimaki, Makoto
N1 - Funding Information:
Acknowledgment This work was partly supported by JSPS KAKENHI Grant Number 25246033.
Publisher Copyright:
© 2018 The Japan Society of Applied Physics.
PY - 2018/3
Y1 - 2018/3
N2 - We have developed an optical disk system for imaging transmitted light from Escherichia coli dispersed on an optical disk. When E. coli was stained using Bismarck brown, the transmittance was found to decrease in images obtained at λ = 405 nm. The results indicate that transmittance imaging is suitable for finding the difference in light intensity between stained and unstained E. coli, whereas the reflectance images were scarcely changed by staining. Therefore, E. coli can be selectively discriminated from abiotic contaminants using transmittance imaging.
AB - We have developed an optical disk system for imaging transmitted light from Escherichia coli dispersed on an optical disk. When E. coli was stained using Bismarck brown, the transmittance was found to decrease in images obtained at λ = 405 nm. The results indicate that transmittance imaging is suitable for finding the difference in light intensity between stained and unstained E. coli, whereas the reflectance images were scarcely changed by staining. Therefore, E. coli can be selectively discriminated from abiotic contaminants using transmittance imaging.
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U2 - 10.7567/APEX.11.037001
DO - 10.7567/APEX.11.037001
M3 - Article
AN - SCOPUS:85044970461
SN - 1882-0778
VL - 11
JO - Applied Physics Express
JF - Applied Physics Express
IS - 3
M1 - 037001
ER -