Selective encapsulation of hemoproteins from mammalian cells using mesoporous metal oxide nanoparticles

Mohamed Khairy, Sherif A. El-Safty*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    11 Citations (Scopus)


    A key requirement in successful protein encapsulation is the fabrication of selective protein supercaptors that are not impeded by the physical shape and three-dimensional hydrodynamics of the protein, exhibit minimal clogging effect but with high protein retention, and with uniformly sized adsorbent pores. We report a novel nanomagnet-selective supercaptor approach in the encapsulation of hemoprotein from mammalian cells using mesoporous metal oxide nanoparticles (NPs). Different morphologies of mesoporous NiO and Fe3O4 NPs were fabricated. Among these nanoadsorbents, NiO nanoroses (NRs) had higher loading capacity of hemoprotein than NiO nanospheres (NSs) and nanoplatelets (NPLs), or even superparamagnetic Fe3O4 NPs. The key finding of this study was that mesoporous NiO nanomagnet supercaptors show exceptional encapsulation and selective separation of high-concentration Hb from human blood. In this induced-fit separation model, in addition to the heme group distributions and protein-carrier binding energy, the morphology and magnetic properties of NiO NPs had a key function in broadening the controlled immobilization affinity and selectivity of hemoproteins. In addition, thermodynamics, kinetics, and theoretical studies were carried out to investigate the optimal performance of protein adsorption.

    Original languageEnglish
    Pages (from-to)460-468
    Number of pages9
    JournalColloids and Surfaces B: Biointerfaces
    Publication statusPublished - 2013 Nov 1


    • Hemoproteins
    • Mammalian cells
    • Mesoporous
    • Metal oxides
    • Selective encapsulation

    ASJC Scopus subject areas

    • Biotechnology
    • Colloid and Surface Chemistry
    • Physical and Theoretical Chemistry
    • Surfaces and Interfaces


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