Selective photoinhibition of photosystem I in isolated thylakoid membranes from cucumber and spinach

Kintake Sonoike*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

89 Citations (Scopus)


The site of photoinhibition at low temperatures in leaves of a chilling-sensitive plant, cucumber, is photosystem I [Terashima et al. (1994) Planta 193: 300]. As described herein, selective photoinhibition of PSI can also be induced in isolated thylakoid membranes in vitro. Inhibition was observed both at chilling temperatures and at 25°C, and not only in the thylakoid membranes isolated from cucumber, but also in those isolated from a chilling-tolerant plant, spinach. Comparison of these observations in vitro to the earlier results in vivo indicates that (1) photoinhibition of PSI is a universal phenomenon; (2) a mechanism exists to protect PSI in vivo; and (3) the protective mechanism is chilling-sensitive in cucumber. The chilling-sensitive component seems to be lost during the isolation of thylakoid membranes. Very weak light (10-20μmol m-2 s-1) was sufficient to cause the inhibition of PSI. About 80% of the oxygen-evolving activity by PSII was maintained even after the activity of PSI had decreased by more than 70%. This is the first report of the selective photoinhibition of PSI in vitro.

Original languageEnglish
Pages (from-to)825-830
Number of pages6
JournalPlant and Cell Physiology
Issue number5
Publication statusPublished - 1995 Jul
Externally publishedYes


  • Active oxygen species
  • Chilling injury
  • Photoinhibition
  • Photosynthesis
  • Photosystem I
  • Stress effect

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology


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