Selectivity improvement in protein nanopatterning with a hydroxy-terminated self-assembled monolayer template

Takeo Miyake; Takashi Tanii; Koichi Kato; Tamotsu Zako; Takashi Funatsu; Iwao Ohdomari

doi:10.1088/0957-4484/18/30/305304

Selectivity improvement in protein nanopatterning with a hydroxy-terminated self-assembled monolayer template

Takeo Miyake^*, Takashi Tanii, Koichi Kato, Tamotsu Zako, Takashi Funatsu, Iwao Ohdomari

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Protein nanopatterning techniques to fulfil the requirement for reducing nonspecific adsorption are demonstrated. A target protein is selectively immobilized on a hydroxy-terminated self-assembled monolayer template, the pattern of which is modified with streptavidin used as the intermediating molecule. 3-aminopropyltrimethoxysilane (APTES) used as the intermediating molecule induces nonspecific adsorption of proteins due to nonspecific adsorption of APTES itself. The data indicate that reducing nonspecific adsorption of both the target protein and the intermediating molecule is important for selectivity improvement in protein patterning. As a result of the refinement, a protein nanopattern of 250 nm in diameter has been fabricated.

Original language	English
Article number	305304
Journal	Nanotechnology
Volume	18
Issue number	30
DOIs	https://doi.org/10.1088/0957-4484/18/30/305304
Publication status	Published - 2007 Aug 8

ASJC Scopus subject areas

Bioengineering
Chemistry(all)
Materials Science(all)
Mechanics of Materials
Mechanical Engineering
Electrical and Electronic Engineering

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AU - Miyake, Takeo

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AU - Funatsu, Takashi

AU - Ohdomari, Iwao

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AB - Protein nanopatterning techniques to fulfil the requirement for reducing nonspecific adsorption are demonstrated. A target protein is selectively immobilized on a hydroxy-terminated self-assembled monolayer template, the pattern of which is modified with streptavidin used as the intermediating molecule. 3-aminopropyltrimethoxysilane (APTES) used as the intermediating molecule induces nonspecific adsorption of proteins due to nonspecific adsorption of APTES itself. The data indicate that reducing nonspecific adsorption of both the target protein and the intermediating molecule is important for selectivity improvement in protein patterning. As a result of the refinement, a protein nanopattern of 250 nm in diameter has been fabricated.

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Selectivity improvement in protein nanopatterning with a hydroxy-terminated self-assembled monolayer template

Abstract

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