Self-sustained circadian rhythm in cultured human mononuclear cells isolated from peripheral blood

Takashi Ebisawa; Kahori Numazawa; Hiroko Shimada; Hiroyuki Izutsu; Tsukasa Sasaki; Nobumasa Kato; Katsushi Tokunaga; Akio Mori; Ken ichi Honma; Sato Honma; Shigenobu Shibata

doi:10.1016/j.neures.2009.10.007

Self-sustained circadian rhythm in cultured human mononuclear cells isolated from peripheral blood

Takashi Ebisawa^*, Kahori Numazawa, Hiroko Shimada, Hiroyuki Izutsu, Tsukasa Sasaki, Nobumasa Kato, Katsushi Tokunaga, Akio Mori, Ken ichi Honma, Sato Honma, Shigenobu Shibata

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Disturbed circadian rhythmicity is associated with human diseases such as sleep and mood disorders. However, study of human endogenous circadian rhythm is laborious and time-consuming, which hampers the elucidation of diseases. It has been reported that peripheral tissues exhibit circadian rhythmicity as the suprachiasmatic nucleus-the center of the biological clock. We tried to study human circadian rhythm using cultured peripheral blood mononuclear cells (PBMCs) obtained from a single collection of venous blood. Activated human PBMCs showed self-sustained circadian rhythm of clock gene expression, which indicates that they are useful for investigating human endogenous circadian rhythm.

Original language	English
Pages (from-to)	223-227
Number of pages	5
Journal	Neuroscience Research
Volume	66
Issue number	2
DOIs	https://doi.org/10.1016/j.neures.2009.10.007
Publication status	Published - 2010 Feb

Keywords

Clock genes
Human biological clock
Human circadian rhythm
Luciferase
Mononuclear cells
Self-sustained circadian rhythm

ASJC Scopus subject areas

Neuroscience(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.neures.2009.10.007

Cite this

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title = "Self-sustained circadian rhythm in cultured human mononuclear cells isolated from peripheral blood",

abstract = "Disturbed circadian rhythmicity is associated with human diseases such as sleep and mood disorders. However, study of human endogenous circadian rhythm is laborious and time-consuming, which hampers the elucidation of diseases. It has been reported that peripheral tissues exhibit circadian rhythmicity as the suprachiasmatic nucleus-the center of the biological clock. We tried to study human circadian rhythm using cultured peripheral blood mononuclear cells (PBMCs) obtained from a single collection of venous blood. Activated human PBMCs showed self-sustained circadian rhythm of clock gene expression, which indicates that they are useful for investigating human endogenous circadian rhythm.",

keywords = "Clock genes, Human biological clock, Human circadian rhythm, Luciferase, Mononuclear cells, Self-sustained circadian rhythm",

author = "Takashi Ebisawa and Kahori Numazawa and Hiroko Shimada and Hiroyuki Izutsu and Tsukasa Sasaki and Nobumasa Kato and Katsushi Tokunaga and Akio Mori and Honma, {Ken ichi} and Sato Honma and Shigenobu Shibata",

note = "Funding Information: We thank Dr Hajime Tei for the permission to use the LM-2400. This work was supported financially by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology.",

year = "2010",

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doi = "10.1016/j.neures.2009.10.007",

language = "English",

volume = "66",

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T1 - Self-sustained circadian rhythm in cultured human mononuclear cells isolated from peripheral blood

AU - Ebisawa, Takashi

AU - Numazawa, Kahori

AU - Shimada, Hiroko

AU - Izutsu, Hiroyuki

AU - Sasaki, Tsukasa

AU - Kato, Nobumasa

AU - Tokunaga, Katsushi

AU - Mori, Akio

AU - Honma, Ken ichi

AU - Honma, Sato

AU - Shibata, Shigenobu

N1 - Funding Information: We thank Dr Hajime Tei for the permission to use the LM-2400. This work was supported financially by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology.

PY - 2010/2

Y1 - 2010/2

N2 - Disturbed circadian rhythmicity is associated with human diseases such as sleep and mood disorders. However, study of human endogenous circadian rhythm is laborious and time-consuming, which hampers the elucidation of diseases. It has been reported that peripheral tissues exhibit circadian rhythmicity as the suprachiasmatic nucleus-the center of the biological clock. We tried to study human circadian rhythm using cultured peripheral blood mononuclear cells (PBMCs) obtained from a single collection of venous blood. Activated human PBMCs showed self-sustained circadian rhythm of clock gene expression, which indicates that they are useful for investigating human endogenous circadian rhythm.

AB - Disturbed circadian rhythmicity is associated with human diseases such as sleep and mood disorders. However, study of human endogenous circadian rhythm is laborious and time-consuming, which hampers the elucidation of diseases. It has been reported that peripheral tissues exhibit circadian rhythmicity as the suprachiasmatic nucleus-the center of the biological clock. We tried to study human circadian rhythm using cultured peripheral blood mononuclear cells (PBMCs) obtained from a single collection of venous blood. Activated human PBMCs showed self-sustained circadian rhythm of clock gene expression, which indicates that they are useful for investigating human endogenous circadian rhythm.

KW - Clock genes

KW - Human biological clock

KW - Human circadian rhythm

KW - Luciferase

KW - Mononuclear cells

KW - Self-sustained circadian rhythm

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JO - Neuroscience Research

JF - Neuroscience Research

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Self-sustained circadian rhythm in cultured human mononuclear cells isolated from peripheral blood

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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