Abstract
We have developed a microfluidic-based fluorescence-activated droplet sorting system using a thermoreversible gelation polymer (TGP) as a switching material. Compared with the conventional fluorescence-activated cell sorter (FACS), TGP-based fluorescence-activated droplet sorting can be performed using a relatively simple optical setup and enables a highly sensitive detection of the fluorescent target [1, 2]. Using this system, we successfully demonstrated the quick and sufficient isolation of a fluorescently labeled subcellular structure (human artificial chromosome; HAC) from the crude cell homogenate. The results presented here constitute the first demonstration of droplet sorting using TGP. This droplet-based microfluidics platform can complement the conventional techniques in proteomics.
| Original language | English |
|---|---|
| Title of host publication | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1259-1261 |
| Number of pages | 3 |
| ISBN (Print) | 9780979806476 |
| Publication status | Published - 2014 |
| Event | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: 2014 Oct 26 → 2014 Oct 30 |
Other
| Other | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
|---|---|
| Country/Territory | United States |
| City | San Antonio |
| Period | 14/10/26 → 14/10/30 |
Keywords
- Droplet sorting
- On-chip sorting
- Proteomics
- Thermoreversible gelation polymer
ASJC Scopus subject areas
- Control and Systems Engineering