Sequence-Tagged NotI Sites of Human Chromosome 21: Sequence Analysis and Mapping

Masahira Hattori; Atushi Toyoda; Hitoshi Ichikawa; Takashi Ito; Hideko Ohgusu; Nobue Oishi; Takeshi Kano; Satoru Kuhara; Misao Ohki; Yoshiyuki Sakaki

doi:10.1006/geno.1993.1280

Sequence-Tagged NotI Sites of Human Chromosome 21: Sequence Analysis and Mapping

Masahira Hattori^*, Atushi Toyoda, Hitoshi Ichikawa, Takashi Ito, Hideko Ohgusu, Nobue Oishi, Takeshi Kano, Satoru Kuhara, Misao Ohki, Yoshiyuki Sakaki

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

We isolated and analyzed 19 NotI site-containing clones specific for human chromosome 21. The overall process consisted of selective isolation of Not I site-containing clones from flow-sorted chromosome 21 libraries, selection of independent clones by their restriction patterns and nucleotide sequences, and assignment of the clones to chromosome 21. Sequence analysis showed that the regions around the NotI sites had features typical of CpG islands, such as extremely high GC content, high-frequency appearance of CpG dinucleotide sequences, and lack of methylation of these CpGs. PCR conditions for these extremely G+C-rich templates were optimized to establish these NotI sites as sequence-tagged sites.

Original language	English
Pages (from-to)	39-44
Number of pages	6
Journal	Genomics
Volume	17
Issue number	1
DOIs	https://doi.org/10.1006/geno.1993.1280
Publication status	Published - 1993 Jul
Externally published	Yes

ASJC Scopus subject areas

Genetics

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abstract = "We isolated and analyzed 19 NotI site-containing clones specific for human chromosome 21. The overall process consisted of selective isolation of Not I site-containing clones from flow-sorted chromosome 21 libraries, selection of independent clones by their restriction patterns and nucleotide sequences, and assignment of the clones to chromosome 21. Sequence analysis showed that the regions around the NotI sites had features typical of CpG islands, such as extremely high GC content, high-frequency appearance of CpG dinucleotide sequences, and lack of methylation of these CpGs. PCR conditions for these extremely G+C-rich templates were optimized to establish these NotI sites as sequence-tagged sites.",

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AU - Hattori, Masahira

AU - Toyoda, Atushi

AU - Ichikawa, Hitoshi

AU - Ito, Takashi

AU - Ohgusu, Hideko

AU - Oishi, Nobue

AU - Kano, Takeshi

AU - Kuhara, Satoru

AU - Ohki, Misao

AU - Sakaki, Yoshiyuki

PY - 1993/7

Y1 - 1993/7

N2 - We isolated and analyzed 19 NotI site-containing clones specific for human chromosome 21. The overall process consisted of selective isolation of Not I site-containing clones from flow-sorted chromosome 21 libraries, selection of independent clones by their restriction patterns and nucleotide sequences, and assignment of the clones to chromosome 21. Sequence analysis showed that the regions around the NotI sites had features typical of CpG islands, such as extremely high GC content, high-frequency appearance of CpG dinucleotide sequences, and lack of methylation of these CpGs. PCR conditions for these extremely G+C-rich templates were optimized to establish these NotI sites as sequence-tagged sites.

AB - We isolated and analyzed 19 NotI site-containing clones specific for human chromosome 21. The overall process consisted of selective isolation of Not I site-containing clones from flow-sorted chromosome 21 libraries, selection of independent clones by their restriction patterns and nucleotide sequences, and assignment of the clones to chromosome 21. Sequence analysis showed that the regions around the NotI sites had features typical of CpG islands, such as extremely high GC content, high-frequency appearance of CpG dinucleotide sequences, and lack of methylation of these CpGs. PCR conditions for these extremely G+C-rich templates were optimized to establish these NotI sites as sequence-tagged sites.

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Sequence-Tagged NotI Sites of Human Chromosome 21: Sequence Analysis and Mapping

Abstract

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