Serine tRNA complementary to the nonuniversal serine codon CUG in Candida cylindracea: Evolutionary implications

T. Yokogawa; T. Suzuki; T. Ueda; M. Mori; T. Ohama; Y. Kuchino; S. Yoshinari; I. Motoki; K. Nishikawa; S. Osawa; K. Watanabe

doi:10.1073/pnas.89.16.7408

Serine tRNA complementary to the nonuniversal serine codon CUG in Candida cylindracea: Evolutionary implications

T. Yokogawa, T. Suzuki, T. Ueda^*, M. Mori, T. Ohama, Y. Kuchino, S. Yoshinari, I. Motoki, K. Nishikawa, S. Osawa, K. Watanabe

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

50 Citations (Scopus)

Abstract

In the asporogenic yeast Candida cylindracea, the codon CUG is read as serine instead of leucine. This is an unusual instance in which the amino acid assignment of a codon deviates from the universal code. To infer the evolutionary process of this change, the tRNA with the anticodon sequence CAG, which is complementary to and thus responsible for translation of the codon CUG, has been identified. Indeed, this tRNA translates an in-frame CUG codon in a synthetic mRNA as serine in an in vitro translation system. The gene for the tRNA is interrupted by an intron in the anticodon loop. Sequence comparisons of the tRNA and its gene suggest that a single cytidine was inserted into the anticodon loop of the gene for tRNA(Ser)IGA during evolution to produce tRNA(Ser)CAG. The tRNA(Ser)CAG may be produced from its precursor molecule containing the cytidine insertion by splicing.

Original language	English
Pages (from-to)	7408-7411
Number of pages	4
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	89
Issue number	16
DOIs	https://doi.org/10.1073/pnas.89.16.7408
Publication status	Published - 1992
Externally published	Yes

Keywords

codon capture
directional mutation pressure
genetic code
intron

ASJC Scopus subject areas

General

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10.1073/pnas.89.16.7408

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Yokogawa, T., Suzuki, T., Ueda, T., Mori, M., Ohama, T., Kuchino, Y., Yoshinari, S., Motoki, I., Nishikawa, K., Osawa, S., & Watanabe, K. (1992). Serine tRNA complementary to the nonuniversal serine codon CUG in Candida cylindracea: Evolutionary implications. Proceedings of the National Academy of Sciences of the United States of America, 89(16), 7408-7411. https://doi.org/10.1073/pnas.89.16.7408

Yokogawa, T, Suzuki, T, Ueda, T, Mori, M, Ohama, T, Kuchino, Y, Yoshinari, S, Motoki, I, Nishikawa, K, Osawa, S & Watanabe, K 1992, 'Serine tRNA complementary to the nonuniversal serine codon CUG in Candida cylindracea: Evolutionary implications', Proceedings of the National Academy of Sciences of the United States of America, vol. 89, no. 16, pp. 7408-7411. https://doi.org/10.1073/pnas.89.16.7408

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abstract = "In the asporogenic yeast Candida cylindracea, the codon CUG is read as serine instead of leucine. This is an unusual instance in which the amino acid assignment of a codon deviates from the universal code. To infer the evolutionary process of this change, the tRNA with the anticodon sequence CAG, which is complementary to and thus responsible for translation of the codon CUG, has been identified. Indeed, this tRNA translates an in-frame CUG codon in a synthetic mRNA as serine in an in vitro translation system. The gene for the tRNA is interrupted by an intron in the anticodon loop. Sequence comparisons of the tRNA and its gene suggest that a single cytidine was inserted into the anticodon loop of the gene for tRNA(Ser)IGA during evolution to produce tRNA(Ser)CAG. The tRNA(Ser)CAG may be produced from its precursor molecule containing the cytidine insertion by splicing.",

keywords = "codon capture, directional mutation pressure, genetic code, intron",

author = "T. Yokogawa and T. Suzuki and T. Ueda and M. Mori and T. Ohama and Y. Kuchino and S. Yoshinari and I. Motoki and K. Nishikawa and S. Osawa and K. Watanabe",

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doi = "10.1073/pnas.89.16.7408",

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T1 - Serine tRNA complementary to the nonuniversal serine codon CUG in Candida cylindracea

T2 - Evolutionary implications

AU - Yokogawa, T.

AU - Suzuki, T.

AU - Ueda, T.

AU - Mori, M.

AU - Ohama, T.

AU - Kuchino, Y.

AU - Yoshinari, S.

AU - Motoki, I.

AU - Nishikawa, K.

AU - Osawa, S.

AU - Watanabe, K.

PY - 1992

Y1 - 1992

N2 - In the asporogenic yeast Candida cylindracea, the codon CUG is read as serine instead of leucine. This is an unusual instance in which the amino acid assignment of a codon deviates from the universal code. To infer the evolutionary process of this change, the tRNA with the anticodon sequence CAG, which is complementary to and thus responsible for translation of the codon CUG, has been identified. Indeed, this tRNA translates an in-frame CUG codon in a synthetic mRNA as serine in an in vitro translation system. The gene for the tRNA is interrupted by an intron in the anticodon loop. Sequence comparisons of the tRNA and its gene suggest that a single cytidine was inserted into the anticodon loop of the gene for tRNA(Ser)IGA during evolution to produce tRNA(Ser)CAG. The tRNA(Ser)CAG may be produced from its precursor molecule containing the cytidine insertion by splicing.

AB - In the asporogenic yeast Candida cylindracea, the codon CUG is read as serine instead of leucine. This is an unusual instance in which the amino acid assignment of a codon deviates from the universal code. To infer the evolutionary process of this change, the tRNA with the anticodon sequence CAG, which is complementary to and thus responsible for translation of the codon CUG, has been identified. Indeed, this tRNA translates an in-frame CUG codon in a synthetic mRNA as serine in an in vitro translation system. The gene for the tRNA is interrupted by an intron in the anticodon loop. Sequence comparisons of the tRNA and its gene suggest that a single cytidine was inserted into the anticodon loop of the gene for tRNA(Ser)IGA during evolution to produce tRNA(Ser)CAG. The tRNA(Ser)CAG may be produced from its precursor molecule containing the cytidine insertion by splicing.

KW - codon capture

KW - directional mutation pressure

KW - genetic code

KW - intron

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Serine tRNA complementary to the nonuniversal serine codon CUG in Candida cylindracea: Evolutionary implications

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Keywords

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