Sex differences in steroidogenesis in skeletal muscle following a single bout of exercise in rats

Katsuji Aizawa*, Motoyuki Iemitsu, Takeshi Otsuki, Seiji Maeda, Takashi Miyauchi, Noboru Mesaki

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

60 Citations (Scopus)


Sex steroid hormones, such as testosterone and estradiol, play important roles in developing both strength and mass of skeletal muscle. Recently, we demonstrated that skeletal muscle can synthesize sex steroid hormones. Whether there are sex differences in basal steroidogenesis or acute exercise-induced alterations of steroidogenesis in the skeletal muscle is unknown. We examined sex differences in the levels of testosterone, estradiol, and steroidogenesis-related enzymes, such as 17β-hydroxysteroid dehydrogenase (HSD), 3β-HSD, and aromatase cytochrome P-450 (P450arom), in the skeletal muscle at rest and after exercise. We studied the gastrocnemius muscles of resting rats (10 wk old) and exercised rats (10 wk old, treadmill running, 30 m/min, 30 min). Basal muscular testosterone levels were higher in males than females, whereas estradiol did not differ between sexes. Additionally, 17β-HSD, 3β-HSD, and P450arom transcript and protein expression were greater in females. After acute exercise, testosterone levels and 17β-HSD expression increased in muscle in both sexes. By comparison, muscular estradiol levels increased in males following exercise but were unchanged in females. Expression of P450arom, which regulates estrogen synthesis, increased after acute exercise in males but decreased after exercise in females. Thus a single bout of exercise can influence the steroidogenic system in skeletal muscle, and these alterations differ between sexes. The acute exercise-induced alteration of steroidogenic enzymes may enhance the local steroidogenesis in the skeletal muscle in both sexes.

Original languageEnglish
Pages (from-to)67-74
Number of pages8
JournalJournal of Applied Physiology
Issue number1
Publication statusPublished - 2008 Jan
Externally publishedYes


  • 17β-hydroxysteroid dehydrogenase
  • 3β-hydroxysteroid dehydrogenase
  • Acute exercise
  • Aromatase cytochrome P-450
  • Sex steroid hormone

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)


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