Abstract
In this study, a simple method for quantitative PCR (qPCR) with a microfluidic device is proposed and evaluated. PCR was performed in a flow-through manner with a SYBR green containing PCR mixture. During and after the PCR operations, the fluorescence intensity was continuously monitored to obtain forward and inversed amplification curves. As a result of evaluation, quite linear calibration curve was obtained both with the forward and the inversed amplification curve.
| Original language | English |
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| Pages | 1743-1745 |
| Number of pages | 3 |
| Publication status | Published - 2008 Jan 1 |
| Event | 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: 2008 Oct 12 → 2008 Oct 16 |
Conference
| Conference | 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 |
|---|---|
| Country/Territory | United States |
| City | San Diego, CA |
| Period | 08/10/12 → 08/10/16 |
Keywords
- Flow-through polymerase chain reaction
- Polydimethylsiloxane
- Quantitative polymerase chain reaction
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering