TY - GEN
T1 - Single cell analysis of the proinflammatory responses of mast cell by a real time secretion assay
AU - Shirasaki, Y.
AU - Nakahara, A.
AU - Shimura, N.
AU - Suzuki, N.
AU - Yamagishi, M.
AU - Mizuno, J.
AU - Shoji, S.
AU - Ohara, O.
PY - 2011
Y1 - 2011
N2 - A real-time secretion monitoring assay from living single-cells was realized by total internal reflection fluorescence imaging with an optically optimized microwell array chip fabricated of an amorphous fluoropolymer, CYTOP on the basis of a fluorescence sandwich immunoassay. After individual mast cells were stimulated with lipopolysaccharide in the microwell array, their proinflammatory responses were monitored as cytokine secretion along time. mRNA levels of cytokines by single-cell quantitative PCR was also measured after the secretion assay. The results showed that each of stimulated mast cells is quantitatively and qualitatively distinguishable from each other in terms of cytokine secretion and their mRNA profile.
AB - A real-time secretion monitoring assay from living single-cells was realized by total internal reflection fluorescence imaging with an optically optimized microwell array chip fabricated of an amorphous fluoropolymer, CYTOP on the basis of a fluorescence sandwich immunoassay. After individual mast cells were stimulated with lipopolysaccharide in the microwell array, their proinflammatory responses were monitored as cytokine secretion along time. mRNA levels of cytokines by single-cell quantitative PCR was also measured after the secretion assay. The results showed that each of stimulated mast cells is quantitatively and qualitatively distinguishable from each other in terms of cytokine secretion and their mRNA profile.
KW - CYTOP
KW - Proinflammatory response
KW - Secretion monitoring assay
KW - Single-cell
UR - http://www.scopus.com/inward/record.url?scp=84883799786&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883799786&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84883799786
SN - 9781618395955
T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
SP - 843
EP - 845
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Y2 - 2 October 2011 through 6 October 2011
ER -