TY - JOUR
T1 - Single-cell observation of phagocytosis by human blood dendritic cells
AU - Ishimoto, Hiroshi
AU - Yanagihara, Katsunori
AU - Araki, Nobuko
AU - Mukae, Hiroshi
AU - Sakamoto, Noriho
AU - Izumikawa, Koichi
AU - Seki, Masafumi
AU - Miyazaki, Yoshitsugu
AU - Hirakata, Yoichi
AU - Mizuta, Yohei
AU - Yasuda, Kenji
AU - Kohno, Shigeru
PY - 2008/8/11
Y1 - 2008/8/11
N2 - Time-lapse video microscopic observation is useful for analysis of cell biology, especially in rapid response of immune cells. Dendritic cells (DCs) have multiple functions in the immune system, and DCs in peripheral blood play an especially important role at the front line of infection. We have developed a time-lapse video microscopic method for the evaluation of single myeloid DCs (MDC-1) from human peripheral blood. MDC-1 displayed generalized plasma membrane ruffling and phagocytosis of Pseudomonas aeruginosa. The morphological changes of MDC-1 increased following stimulation with P. aeruginosa but not after stimulation with supernatant from a P. aeruginosa culture. The activation of these morphological changes in MDC-1 could be quantitatively analyzed using the time-lapse video microscopy. This novel system may be useful for the evaluation of rapid response with human immune cells against bacterial infection.
AB - Time-lapse video microscopic observation is useful for analysis of cell biology, especially in rapid response of immune cells. Dendritic cells (DCs) have multiple functions in the immune system, and DCs in peripheral blood play an especially important role at the front line of infection. We have developed a time-lapse video microscopic method for the evaluation of single myeloid DCs (MDC-1) from human peripheral blood. MDC-1 displayed generalized plasma membrane ruffling and phagocytosis of Pseudomonas aeruginosa. The morphological changes of MDC-1 increased following stimulation with P. aeruginosa but not after stimulation with supernatant from a P. aeruginosa culture. The activation of these morphological changes in MDC-1 could be quantitatively analyzed using the time-lapse video microscopy. This novel system may be useful for the evaluation of rapid response with human immune cells against bacterial infection.
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M3 - Article
C2 - 18653972
AN - SCOPUS:48749092608
SN - 1344-6304
VL - 61
SP - 294
EP - 297
JO - Japanese Journal of Infectious Diseases
JF - Japanese Journal of Infectious Diseases
IS - 4
ER -
