From human chromosome 21-specific libraries, 22 SfiI linking clones and 38 P1 clones were isolated and regionally mapped on the chromosome. The terminal sequences of these clones were determined and pairs of PCR primers were generated which could specifically amplify the sequenced regions. These sequence-tagged sites (STSs) should be useful for constructing a high resolution map of human chromosome 21.
|Number of pages
|Published - 1994
- Human chromosome restriction enzyme SfiI
- Linking clone
- P1 clone, high-resolution physical map
ASJC Scopus subject areas
- Molecular Biology