@article{4f669943a07d459f9d33d62564f52186,
title = "Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization",
abstract = "Ribosome biogenesis is a fundamental multi-step cellular process that culminates in the formation of ribosomal subunits, whose production and modification are regulated by numerous biogenesis factors. In this study, we analyze physiologic prokaryotic ribosome biogenesis by isolating bona fide pre-50S subunits from an Escherichia coli strain with the biogenesis factor ObgE, affinity tagged at its native gene locus. Our integrative structural approach reveals a network of interacting biogenesis factors consisting of YjgA, RluD, RsfS, and ObgE on the immature pre-50S subunit. In addition, our study provides mechanistic insight into how the GTPase ObgE, in concert with other biogenesis factors, facilitates the maturation of the 50S functional core and reveals both conserved and divergent evolutionary features of ribosome biogenesis between prokaryotes and eukaryotes.",
keywords = "50S subunit, ObgE, RluD, RsfS, YjgA, cryo-electron microscopy, ribosome, ribosome assembly, ribosome biogenesis, structure",
author = "Rainer Nikolay and Tarek Hilal and Sabine Schmidt and Bo Qin and David Schwefel and Vieira-Vieira, {Carlos H.} and Thorsten Mielke and J{\"o}rg B{\"u}rger and Justus Loerke and Kazuaki Amikura and Timo Fl{\"u}gel and Takuya Ueda and Matthias Selbach and Elke Deuerling and Spahn, {Christian M.T.}",
note = "Funding Information: We would like to thank Anett Unbehaun and Matthew Kraushar for profound scientific discussions and text editing. This work was funded by Konstanz Research School Chemical Biology (to S.S.), the Human Frontier Science Program Organization ( HFSP-Ref. RPG0008/2014 , to C.M.T.S.), and Deutsche Forschungsgemeinschaft DFG grants FOR1805 and SFB740 (to C.M.T.S. and T.M.), DFG grant/SFB969 project A01 (to E.D.), DFG Emmy Noether Programme SCHW1851/1-1 (to D.S.), and a European Molecular Biology Organization EMBO Advanced laboratory start-up grant aALTF-1650 (to D.S.). The authors acknowledge the North-German Supercomputing Alliance (HLRN, beb00007 ) for providing HPC resources that have contributed to the research results reported in this paper. We acknowledge access to electron microscopic equipment at the core facility BioSupraMol of Freie Universit{\"a}t Berlin, supported through grants from the Deutsche Forschungsgemeinschaft and the State of Berlin for large equipment according to Art. 91b GG ( INST 335/588-1 FUGG , INST 335/589-1 FUGG , and INST 335/590-1 FUGG ). Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",
year = "2021",
month = mar,
day = "18",
doi = "10.1016/j.molcel.2021.02.006",
language = "English",
volume = "81",
pages = "1200--1215.e9",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "6",
}