Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization

Rainer Nikolay*, Tarek Hilal, Sabine Schmidt, Bo Qin, David Schwefel, Carlos H. Vieira-Vieira, Thorsten Mielke, Jörg Bürger, Justus Loerke, Kazuaki Amikura, Timo Flügel, Takuya Ueda, Matthias Selbach, Elke Deuerling, Christian M.T. Spahn*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)


Ribosome biogenesis is a fundamental multi-step cellular process that culminates in the formation of ribosomal subunits, whose production and modification are regulated by numerous biogenesis factors. In this study, we analyze physiologic prokaryotic ribosome biogenesis by isolating bona fide pre-50S subunits from an Escherichia coli strain with the biogenesis factor ObgE, affinity tagged at its native gene locus. Our integrative structural approach reveals a network of interacting biogenesis factors consisting of YjgA, RluD, RsfS, and ObgE on the immature pre-50S subunit. In addition, our study provides mechanistic insight into how the GTPase ObgE, in concert with other biogenesis factors, facilitates the maturation of the 50S functional core and reveals both conserved and divergent evolutionary features of ribosome biogenesis between prokaryotes and eukaryotes.

Original languageEnglish
Pages (from-to)1200-1215.e9
JournalMolecular Cell
Issue number6
Publication statusPublished - 2021 Mar 18
Externally publishedYes


  • 50S subunit
  • ObgE
  • RluD
  • RsfS
  • YjgA
  • cryo-electron microscopy
  • ribosome
  • ribosome assembly
  • ribosome biogenesis
  • structure

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization'. Together they form a unique fingerprint.

Cite this