SNP detection in transforming growth factor-β1 gene using bacterial magnetic particles

Hiroyuki Ota; Haruko Takeyama; Hideki Nakayama; Takahiko Katoh; Tadashi Matsunaga

doi:10.1016/S0956-5663(03)00015-0

SNP detection in transforming growth factor-β1 gene using bacterial magnetic particles

Hiroyuki Ota, Haruko Takeyama, Hideki Nakayama, Takahiko Katoh, Tadashi Matsunaga^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

A single nucleotide polymorphism (SNP) within the transforming growth factor-β1 (TGF-β1) gene was detected by hybridization-based method using bacterial magnetic particles (BMPs). TGF-β1 is commonly associated with a single base change resulting in a Leu¹⁰→Pro (T⁸⁶⁹→C) polymorphism and is a genetic marker for susceptibility to osteoporosis. Short (9 bases) and specific probes were designed to detect SNP in TGF-β1. Detection probes were immobilized on BMPs using cross-linking reagents. TGF-β1 PCR products (139 bp) were labeled with the fluorescent dye coumarin and hybridized with detection probes on BMPs. Complementary hybridized targets gave over four times higher fluorescent intensities, compared with one base mismatched hybridizations. The SNP genotype was successfully discriminated using this technique.

Original language	English
Pages (from-to)	683-687
Number of pages	5
Journal	Biosensors and Bioelectronics
Volume	18
Issue number	5-6
DOIs	https://doi.org/10.1016/S0956-5663(03)00015-0
Publication status	Published - 2003 May 1
Externally published	Yes

Keywords

Bacterial magnetic particles
Hybridization
Single nucleotide polymorphism
TGF-β1

ASJC Scopus subject areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

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10.1016/S0956-5663(03)00015-0

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title = "SNP detection in transforming growth factor-β1 gene using bacterial magnetic particles",

abstract = "A single nucleotide polymorphism (SNP) within the transforming growth factor-β1 (TGF-β1) gene was detected by hybridization-based method using bacterial magnetic particles (BMPs). TGF-β1 is commonly associated with a single base change resulting in a Leu10→Pro (T869→C) polymorphism and is a genetic marker for susceptibility to osteoporosis. Short (9 bases) and specific probes were designed to detect SNP in TGF-β1. Detection probes were immobilized on BMPs using cross-linking reagents. TGF-β1 PCR products (139 bp) were labeled with the fluorescent dye coumarin and hybridized with detection probes on BMPs. Complementary hybridized targets gave over four times higher fluorescent intensities, compared with one base mismatched hybridizations. The SNP genotype was successfully discriminated using this technique.",

keywords = "Bacterial magnetic particles, Hybridization, Single nucleotide polymorphism, TGF-β1",

author = "Hiroyuki Ota and Haruko Takeyama and Hideki Nakayama and Takahiko Katoh and Tadashi Matsunaga",

note = "Funding Information: This work was funded in part by a Grant-in-Aid for Specially Promoted Research (2), No. 13002005, and Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan.",

year = "2003",

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doi = "10.1016/S0956-5663(03)00015-0",

language = "English",

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T1 - SNP detection in transforming growth factor-β1 gene using bacterial magnetic particles

AU - Ota, Hiroyuki

AU - Takeyama, Haruko

AU - Nakayama, Hideki

AU - Katoh, Takahiko

AU - Matsunaga, Tadashi

N1 - Funding Information: This work was funded in part by a Grant-in-Aid for Specially Promoted Research (2), No. 13002005, and Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan.

PY - 2003/5/1

Y1 - 2003/5/1

N2 - A single nucleotide polymorphism (SNP) within the transforming growth factor-β1 (TGF-β1) gene was detected by hybridization-based method using bacterial magnetic particles (BMPs). TGF-β1 is commonly associated with a single base change resulting in a Leu10→Pro (T869→C) polymorphism and is a genetic marker for susceptibility to osteoporosis. Short (9 bases) and specific probes were designed to detect SNP in TGF-β1. Detection probes were immobilized on BMPs using cross-linking reagents. TGF-β1 PCR products (139 bp) were labeled with the fluorescent dye coumarin and hybridized with detection probes on BMPs. Complementary hybridized targets gave over four times higher fluorescent intensities, compared with one base mismatched hybridizations. The SNP genotype was successfully discriminated using this technique.

AB - A single nucleotide polymorphism (SNP) within the transforming growth factor-β1 (TGF-β1) gene was detected by hybridization-based method using bacterial magnetic particles (BMPs). TGF-β1 is commonly associated with a single base change resulting in a Leu10→Pro (T869→C) polymorphism and is a genetic marker for susceptibility to osteoporosis. Short (9 bases) and specific probes were designed to detect SNP in TGF-β1. Detection probes were immobilized on BMPs using cross-linking reagents. TGF-β1 PCR products (139 bp) were labeled with the fluorescent dye coumarin and hybridized with detection probes on BMPs. Complementary hybridized targets gave over four times higher fluorescent intensities, compared with one base mismatched hybridizations. The SNP genotype was successfully discriminated using this technique.

KW - Bacterial magnetic particles

KW - Hybridization

KW - Single nucleotide polymorphism

KW - TGF-β1

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DO - 10.1016/S0956-5663(03)00015-0

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AN - SCOPUS:0037403911

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JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

IS - 5-6

ER -

SNP detection in transforming growth factor-β1 gene using bacterial magnetic particles

Abstract

Keywords

ASJC Scopus subject areas

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