Some plasma component is essential for IL-6 secretion by neutrophils

Katsutaka Oishi, Kazuhiko Machida*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


Recently, the neutrophil has been shown to have the capacity to produce a number of cytokines. Among these cytokines, interleukin-1 (IL-1), tumor necrosis factor-α (TNF-α) and IL-6 are typical examples of multifunctional cytokines involved in regulation of the immune response and inflammation. However, there are conflicting reports in the literature concerning the issue of whether IL-6 expression can be modulated in human neutrophils. In this study, we showed the spontaneous secretion of IL-1β, TNF-α and IL-6 by neutrophils. IL-6 secretion of neutrophils was effectively induced by IL- 1β. However, spontaneous or IL-1β induced secretion of IL-6 was observed only when the cells were cultured in autologous plasma. Then we examined the spontaneous secretion of cytokines by co-cultured leukocytes (neutrophils, monocytes, and lymphocytes). Surprisingly, IL-6 was dramatically secreted by the leukocytes only when the cells were cultured in autologous plasma, although IL-1β and TNF- α were secreted by the cells either in RPMI 1640 or in plasma. These observations suggest that the regulation of IL-6 production is qualitatively different from the production of IL-1β or TNF- α by leukocytes.

Original languageEnglish
Pages (from-to)89-92
Number of pages4
JournalEnvironmental Health and Preventive Medicine
Issue number2
Publication statusPublished - 1997 Jul


  • IL-1β
  • IL-6
  • Monocytes
  • Neutrophils
  • Plasma

ASJC Scopus subject areas

  • Pollution
  • Public Health, Environmental and Occupational Health


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