Spatiotemporal analysis of localized circadian arrhythmias in plant roots

Naoki Seki, Yusuke Tanigaki, Atsumasa Yoshida, Hirokazu Fukuda*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


The circadian system in plants is characterized by substantial cellular oscillations over an approximately 24-h period. Interactions between cellular oscillators trigger phase resets near the root meristem, resulting in localized regions of arrhythmic expression of the clock gene CCA1. The arrhythmicity of the circadian rhythm significantly impacts physiologic processes and growth; however, the exact nature of these arrhythmic regions remains unknown. In this study, we analyzed spatiotemporal patterns in CCA1 expression in arrhythmic regions using a nondestructive imaging technique. We found that formation of root arrhythmic regions involves the emergence of small spiral waves. Near the small spiral waves, the synchrony of cellular oscillations was low. Our findings provide experimental evidence that the arrhythmias are based on desynchronization of cellular oscillators and enhance our understanding of the role of circadian phenomena in root growth.

Original languageEnglish
Pages (from-to)93-97
Number of pages5
JournalEnvironmental Control in Biology
Issue number3
Publication statusPublished - 2018
Externally publishedYes


  • Arabidopsis thaliana
  • Desynchronization
  • Luciferase bioluminescence assay
  • Phase-reset
  • Spiral wave
  • Stripe wave

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science


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