Spermidine inhibits transient and stable ribosome subunit dissociation

So Umekage, Takuya Ueda*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


Recent light-scattering experiments and sucrose density gradient centrifugational analyses suggested that the 70S ribosome undergoes RRF- and EF-G-triggered transient subunit dissociation that is followed by IF3-induced stable dissociation. However, the experimental conditions did not include the ubiquitous cellular polyamine spermidine, which is required for efficient translation. We found that when spermidine was present, the transient dissociation was inhibited. Moreover, the published experiments used ribosome concentrations that were far lower than the physiological concentration. We found that when spermidine and higher ribosome concentrations were included in the experimental conditions, only very limited stable subunit dissociation was observed. These results suggest that neither transient nor stable dissociation occurs under physiological conditions applied here.

Original languageEnglish
Pages (from-to)1222-1226
Number of pages5
JournalFEBS Letters
Issue number5
Publication statusPublished - 2006 Feb 20
Externally publishedYes


  • Light-scattering
  • Ribosome
  • Spermidine
  • Stable dissociation
  • Sucrose density gradient centrifugation
  • Transient dissociation

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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