Stable Generation of Single- Micron Droplets and Highly Efficient Encapsulation of Cells by Multi-Branch Channels

Seito Shijo; Shuichi Shoji; Daiki Tanaka; Tetsushi Sekiguchi; Masahiro Furuya

doi:10.1109/MEMS51670.2022.9699584

Stable Generation of Single- Micron Droplets and Highly Efficient Encapsulation of Cells by Multi-Branch Channels

Seito Shijo, Shuichi Shoji, Daiki Tanaka, Tetsushi Sekiguchi, Masahiro Furuya

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We have developed an efficient, stable device for generating single-micrometer-scale (1-2 m) droplets based on the fragmentation of droplet tails by tailing. The device, created by a ple soft lithography process, induced continuous droplet fragmentation by branched channels under low-flow conditions (1-10 L/min). The flow rate and surfactant concentration were also important factors for droplet fragmentation. Examining 10 combinations of flow rate and surfactant concentration revealed the optimal conditions, which produced droplets less than 2 m in size at a generation rate of 61.1%. With this method, we efficiently encapsulated cell-mimicking microbeads into passively generated single-micrometer-scale microdroplets.

Original language	English
Title of host publication	35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022
Publisher	IEEE Computer Society
Pages	900-903
Number of pages	4
ISBN (Electronic)	9781665409117
DOIs	https://doi.org/10.1109/MEMS51670.2022.9699584
Publication status	Published - 2022
Event	35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022 - Tokyo, Japan Duration: 2022 Jan 9 → 2022 Jan 13

Publication series

Name	IEEE Symposium on Mass Storage Systems and Technologies
Volume	2022-January
ISSN (Print)	2160-1968

Conference

Conference	35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022
Country/Territory	Japan
City	Tokyo
Period	22/1/9 → 22/1/13

ASJC Scopus subject areas

Hardware and Architecture
Electrical and Electronic Engineering

Access to Document

10.1109/MEMS51670.2022.9699584

Cite this

Shijo, S., Shoji, S., Tanaka, D., Sekiguchi, T., & Furuya, M. (2022). Stable Generation of Single- Micron Droplets and Highly Efficient Encapsulation of Cells by Multi-Branch Channels. In 35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022 (pp. 900-903). (IEEE Symposium on Mass Storage Systems and Technologies; Vol. 2022-January). IEEE Computer Society. https://doi.org/10.1109/MEMS51670.2022.9699584

Stable Generation of Single- Micron Droplets and Highly Efficient Encapsulation of Cells by Multi-Branch Channels. / Shijo, Seito; Shoji, Shuichi; Tanaka, Daiki et al.
35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022. IEEE Computer Society, 2022. p. 900-903 (IEEE Symposium on Mass Storage Systems and Technologies; Vol. 2022-January).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Shijo, S, Shoji, S, Tanaka, D, Sekiguchi, T & Furuya, M 2022, Stable Generation of Single- Micron Droplets and Highly Efficient Encapsulation of Cells by Multi-Branch Channels. in 35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022. IEEE Symposium on Mass Storage Systems and Technologies, vol. 2022-January, IEEE Computer Society, pp. 900-903, 35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022, Tokyo, Japan, 22/1/9. https://doi.org/10.1109/MEMS51670.2022.9699584

Shijo S, Shoji S, Tanaka D, Sekiguchi T, Furuya M. Stable Generation of Single- Micron Droplets and Highly Efficient Encapsulation of Cells by Multi-Branch Channels. In 35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022. IEEE Computer Society. 2022. p. 900-903. (IEEE Symposium on Mass Storage Systems and Technologies). doi: 10.1109/MEMS51670.2022.9699584

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title = "Stable Generation of Single- Micron Droplets and Highly Efficient Encapsulation of Cells by Multi-Branch Channels",

abstract = "We have developed an efficient, stable device for generating single-micrometer-scale (1-2 m) droplets based on the fragmentation of droplet tails by tailing. The device, created by a ple soft lithography process, induced continuous droplet fragmentation by branched channels under low-flow conditions (1-10 L/min). The flow rate and surfactant concentration were also important factors for droplet fragmentation. Examining 10 combinations of flow rate and surfactant concentration revealed the optimal conditions, which produced droplets less than 2 m in size at a generation rate of 61.1%. With this method, we efficiently encapsulated cell-mimicking microbeads into passively generated single-micrometer-scale microdroplets. ",

author = "Seito Shijo and Shuichi Shoji and Daiki Tanaka and Tetsushi Sekiguchi and Masahiro Furuya",

note = "Funding Information: This research was partially funded by Canon Medical Systems Corporation and by a Grant-in-aid for Basic Scientific Research (A) from the Japanese Ministry of Education, Culture, Sports, Science and Technology, grant number 20H00336. Publisher Copyright: {\textcopyright} 2022 IEEE.; 35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022 ; Conference date: 09-01-2022 Through 13-01-2022",

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doi = "10.1109/MEMS51670.2022.9699584",

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AU - Furuya, Masahiro

N1 - Funding Information: This research was partially funded by Canon Medical Systems Corporation and by a Grant-in-aid for Basic Scientific Research (A) from the Japanese Ministry of Education, Culture, Sports, Science and Technology, grant number 20H00336. Publisher Copyright: © 2022 IEEE.

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N2 - We have developed an efficient, stable device for generating single-micrometer-scale (1-2 m) droplets based on the fragmentation of droplet tails by tailing. The device, created by a ple soft lithography process, induced continuous droplet fragmentation by branched channels under low-flow conditions (1-10 L/min). The flow rate and surfactant concentration were also important factors for droplet fragmentation. Examining 10 combinations of flow rate and surfactant concentration revealed the optimal conditions, which produced droplets less than 2 m in size at a generation rate of 61.1%. With this method, we efficiently encapsulated cell-mimicking microbeads into passively generated single-micrometer-scale microdroplets.

AB - We have developed an efficient, stable device for generating single-micrometer-scale (1-2 m) droplets based on the fragmentation of droplet tails by tailing. The device, created by a ple soft lithography process, induced continuous droplet fragmentation by branched channels under low-flow conditions (1-10 L/min). The flow rate and surfactant concentration were also important factors for droplet fragmentation. Examining 10 combinations of flow rate and surfactant concentration revealed the optimal conditions, which produced droplets less than 2 m in size at a generation rate of 61.1%. With this method, we efficiently encapsulated cell-mimicking microbeads into passively generated single-micrometer-scale microdroplets.

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Stable Generation of Single- Micron Droplets and Highly Efficient Encapsulation of Cells by Multi-Branch Channels

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