Stoichiometry of the reaction of oxyhemoglobin with nitrite

Hiroaki Kosaka*, Kazuhiko Imaizumi, Kiyohiro Imai, Itiro Tyuma

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

113 Citations (Scopus)


During the reaction of oxyhemoglobin (HbO2) with nitrite, the concentration of residual nitrite, nitrate, oxygen, and methemoglobin (Hb+) was determined successively. The results obtained at various pH values indicate the following stoichiometry for the overall reaction: 4HbO2 + 4NO- 2 + → 4Hb+ + 4NO- 3 + O2 + 2H2O (Hb denotes hemoglobin monomer). NO2 - binds with methemoglobin noncooperatively with a binding constant of 340 M-1 at pH 7.4 and 25°C. Thus, the major part of Hb+ produced is aquomethemoglobin, not methemoglobin nitrite, when less than 2 equivalents of nitrite is used for the oxidation.

Original languageEnglish
Pages (from-to)184-188
Number of pages5
JournalBBA - Protein Structure
Issue number1
Publication statusPublished - 1979 Nov 23
Externally publishedYes


  • Nitrite
  • Oxyhemoglobin
  • Stoichiometry

ASJC Scopus subject areas

  • Medicine(all)


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