TY - JOUR
T1 - Structural property of DNA that migrates faster in gel electrophoresis, as deduced by CD spectroscopy
AU - Matsugami, Akimasa
AU - Tani, Keiko
AU - Ouhashi, Kiyoshi
AU - Uesugi, Seiichi
AU - Morita, Mitsunori
AU - Ohyama, Takashi
AU - Katahira, Masato
N1 - Funding Information:
Received 26 December 2005; accepted 23 January 2006. We thank Dr. Hideki Tagashira for technical assistance. M.K. was supported by Grants-in-Aid for Scientific Research (Nos. 17026011, 17048009, 18011008, and 18370046) and the Protein 3000 Project of MEXT and by a PRESTO grant of JST. T.O. was supported by MEXT (No. 17050022). A.M. is supported by a postdoctoral fellow program of JSPS. This article is dedicated to Professor Eiko Ohtsuka on the occasion of her 70th birthday. Address correspondence to Masato Katahira, Supramolecular Biology, International Graduate School of Arts and Sciences, Yokohama City University, 1-7-29 Suehiro, Tsurumi-ku, Yokohama 230-0045, Japan. E-mail: katahira@tsurumi.yokohama-cu.ac.jp
PY - 2006/6/1
Y1 - 2006/6/1
N2 - Bent DNAs are known to migrate slower than ordinary DNA in non-denaturing polyacrylamide gel electrophoresis. In contrast, several satellite DNAs have been shown to migrate fast. The structural property that causes the fast migration, however, is not clarified so far on molecular basis. We have investigated the structural property of a satellite DNA, which contains consecutive purine sequences and migrates faster in gel, by CD spectroscopy. Partial formation of an A-form-like structure has been suggested. Reduction in DNA length due to the formation of the A-form-like structure may be responsible for the fast migration. The pronounced rigidity of DNA may also contribute to the behavior.
AB - Bent DNAs are known to migrate slower than ordinary DNA in non-denaturing polyacrylamide gel electrophoresis. In contrast, several satellite DNAs have been shown to migrate fast. The structural property that causes the fast migration, however, is not clarified so far on molecular basis. We have investigated the structural property of a satellite DNA, which contains consecutive purine sequences and migrates faster in gel, by CD spectroscopy. Partial formation of an A-form-like structure has been suggested. Reduction in DNA length due to the formation of the A-form-like structure may be responsible for the fast migration. The pronounced rigidity of DNA may also contribute to the behavior.
KW - A-Form
KW - Bent DNA
KW - CD
KW - Satellite DNA
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U2 - 10.1080/15257770600684068
DO - 10.1080/15257770600684068
M3 - Article
C2 - 16838835
AN - SCOPUS:33747617082
SN - 1525-7770
VL - 25
SP - 417
EP - 425
JO - Nucleosides, Nucleotides and Nucleic Acids
JF - Nucleosides, Nucleotides and Nucleic Acids
IS - 4-6
ER -