Structural uniformity of pullulan produced by several strains of pullularia pullulans

Ryo Taguchi, Yo Kikuchi, Yoshiyuki Sakano, Tsuneo Kobayashi

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66 Citations (Scopus)


Extracellular polysaccharides produced by 3 strains of Pullularia pullulans were fractionated by treating with cetyl trimethylammonium hydroxide into soluble and insoluble fractions, and the structure of the former fraction, i.e., pullulan, was studied. The yield and the ratio of 2 fractions varied widely according to the strains. But the structure of pullulan was found to be uniform irrespective of the strains used. All 3 samples of pullulan gave only glucose on complete acid hydrolysis, and 93-95% maltotriose and 5-7% maltotetraose after isoamylase (pullulanase) action. The ratio of a-1, 4- to a-1, 6-glucosidic linkages calculated from periodate oxidation data coincided very well with the value expected from the ratio of maltotriose to maltotetraose units. An evidence for the complete absence of branch structure in pullulan was presented from the results of hydrolysis by pullulan 4-glucanohydrolase.

Original languageEnglish
Pages (from-to)1583-1588
Number of pages6
JournalAgricultural and Biological Chemistry
Issue number7
Publication statusPublished - 1973
Externally publishedYes

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology
  • General Agricultural and Biological Sciences


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