Structure and Expression of Novel Protein-Tyrosine Kinases, EMB and EMT, in Hematopoietic Cells

Nobuo Yamada, Yuko Kawakami, Hitoshi Kimura, Hiromi Fukamachi, Gottfried Baier, Amnon Altman, Takashi Kato, Yoshimasa Inagaki, Toshiaki Kawakami*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

115 Citations (Scopus)


Two novel tyrosine kinase cDNAs were obtained from murine mast cells. These kinases, Emb and Emt, constitute a novel tyrosine kinase subfamily which may also include Tec, a kinase preferentially expressed in liver, and Dsrc28, a fruit fly kinase. Both lack hydrophobic stretches characteristic of the transmembrane domains found in growth factor receptor tyrosine kinases and carboxyl-terminal, negative regulatory tyrosine residue found in Src family kinases. In addition to the Src homology region 2 (SH2) and SH3 domains characteristic of the Src family kinases and other signaling molecules, Emb and Emt share a similar amino-terminal domain comprised mainly of two repeat segments. The emb 2.7-kb transcript was expressed in mast cells, myeloid cells and B lymphocytes while the emt 4.6-kb mRNA in mast cells, myeloid cells and T lymphocytes. The evidence for in vitro tyrosine kinase activity of Emb and Emt proteins is also provided.

Original languageEnglish
Pages (from-to)231-240
Number of pages10
JournalBiochemical and Biophysical Research Communications
Issue number1
Publication statusPublished - 1993
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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