Structure and solution properties of lipidheme-microsphere

T. Komatsu; Hiroyuki Nishide; E. Tsuchida

Structure and solution properties of lipidheme-microsphere

T. Komatsu^*, Hiroyuki Nishide, E. Tsuchida

^*Corresponding author for this work

Waseda Research Institute for Science and Engineering

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Triglyceride microsphere emulsified with phospholipid derivative of heme (5,10,15,20-tetrakis[α,α,α,α-o-[2,2-dimethyl-20-[2-(trimethylammonioethoxy) phosphonatoxy]eicosanamido]phenyl]porphinatoiron(II); lipidheme) provides a totally synthetic artificial red cell (lipidheme-microsphere; LH-M). Its structure, solution properties and O₂ binding ability are described. The particle diameter of the LH-M was ca. 90 nmφ elucidated by electron microscopy. Viscosity of the LH-M suspension (~1.5 cP) was much lower than that of human blood and the viscosity of mixed system of LH-M/human blood (1/1(v/v)) was 2.5 cP. Specific gravity, osmotic pressure, and colloid osmotic pressure of the LH-M suspension also satisfied the physiological needs. The LH-M can bind O₂ reversibly in response to O₂ pressure (P₅₀(O₂): 41 torr (pH 7.4, 37°C), O₂ solubility of the LH-M was more than that of human blood caused by its high heme concentration.

Original language	English
Pages (from-to)	855-860
Number of pages	6
Journal	Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
Volume	22
Issue number	3
Publication status	Published - 1994

ASJC Scopus subject areas

Biotechnology
Biomedical Engineering
Biomaterials
Hematology

Cite this

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title = "Structure and solution properties of lipidheme-microsphere",

abstract = "Triglyceride microsphere emulsified with phospholipid derivative of heme (5,10,15,20-tetrakis[α,α,α,α-o-[2,2-dimethyl-20-[2-(trimethylammonioethoxy) phosphonatoxy]eicosanamido]phenyl]porphinatoiron(II); lipidheme) provides a totally synthetic artificial red cell (lipidheme-microsphere; LH-M). Its structure, solution properties and O2 binding ability are described. The particle diameter of the LH-M was ca. 90 nmφ elucidated by electron microscopy. Viscosity of the LH-M suspension (~1.5 cP) was much lower than that of human blood and the viscosity of mixed system of LH-M/human blood (1/1(v/v)) was 2.5 cP. Specific gravity, osmotic pressure, and colloid osmotic pressure of the LH-M suspension also satisfied the physiological needs. The LH-M can bind O2 reversibly in response to O2 pressure (P50(O2): 41 torr (pH 7.4, 37°C), O2 solubility of the LH-M was more than that of human blood caused by its high heme concentration.",

author = "T. Komatsu and Hiroyuki Nishide and E. Tsuchida",

year = "1994",

language = "English",

volume = "22",

pages = "855--860",

journal = "Artificial Cells, Blood Substitutes, and Immobilization Biotechnology",

issn = "1073-1199",

publisher = "Informa Healthcare",

number = "3",

}

TY - JOUR

T1 - Structure and solution properties of lipidheme-microsphere

AU - Komatsu, T.

AU - Nishide, Hiroyuki

AU - Tsuchida, E.

PY - 1994

Y1 - 1994

N2 - Triglyceride microsphere emulsified with phospholipid derivative of heme (5,10,15,20-tetrakis[α,α,α,α-o-[2,2-dimethyl-20-[2-(trimethylammonioethoxy) phosphonatoxy]eicosanamido]phenyl]porphinatoiron(II); lipidheme) provides a totally synthetic artificial red cell (lipidheme-microsphere; LH-M). Its structure, solution properties and O2 binding ability are described. The particle diameter of the LH-M was ca. 90 nmφ elucidated by electron microscopy. Viscosity of the LH-M suspension (~1.5 cP) was much lower than that of human blood and the viscosity of mixed system of LH-M/human blood (1/1(v/v)) was 2.5 cP. Specific gravity, osmotic pressure, and colloid osmotic pressure of the LH-M suspension also satisfied the physiological needs. The LH-M can bind O2 reversibly in response to O2 pressure (P50(O2): 41 torr (pH 7.4, 37°C), O2 solubility of the LH-M was more than that of human blood caused by its high heme concentration.

AB - Triglyceride microsphere emulsified with phospholipid derivative of heme (5,10,15,20-tetrakis[α,α,α,α-o-[2,2-dimethyl-20-[2-(trimethylammonioethoxy) phosphonatoxy]eicosanamido]phenyl]porphinatoiron(II); lipidheme) provides a totally synthetic artificial red cell (lipidheme-microsphere; LH-M). Its structure, solution properties and O2 binding ability are described. The particle diameter of the LH-M was ca. 90 nmφ elucidated by electron microscopy. Viscosity of the LH-M suspension (~1.5 cP) was much lower than that of human blood and the viscosity of mixed system of LH-M/human blood (1/1(v/v)) was 2.5 cP. Specific gravity, osmotic pressure, and colloid osmotic pressure of the LH-M suspension also satisfied the physiological needs. The LH-M can bind O2 reversibly in response to O2 pressure (P50(O2): 41 torr (pH 7.4, 37°C), O2 solubility of the LH-M was more than that of human blood caused by its high heme concentration.

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M3 - Article

C2 - 7994409

AN - SCOPUS:0028021484

SN - 1073-1199

VL - 22

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JO - Artificial Cells, Blood Substitutes, and Immobilization Biotechnology

JF - Artificial Cells, Blood Substitutes, and Immobilization Biotechnology

IS - 3

ER -

Structure and solution properties of lipidheme-microsphere

Abstract

ASJC Scopus subject areas

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