Structure of the rat α2-macroglobulin-coding gene

Masahira Hattori; Kusakabe Shin-ichi; Ohgusu Hideko; Tsuchiya Yoshihiro; Ito Takashi; Sakaki Yoshiyuki

doi:10.1016/0378-1119(89)90081-4

Structure of the rat α₂-macroglobulin-coding gene

Masahira Hattori, Kusakabe Shin-ichi, Ohgusu Hideko, Tsuchiya Yoshihiro, Ito Takashi, Sakaki Yoshiyuki^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Rat α₂-macroglobulin (α₂M) is an acute-phase protein i.e., produced upon tissue inflammation. Genomic DNA clones covering the entire sequence of the α₂M gene were isolated and characterized by restriction mapping, Southern blotting and (partial) DNA sequencing. The rat α₂M gene is approx. 50 kb in length and consists of 36 exons ranging in size from 21 to 229 bp. Two functional domains, a bait region and a thiol ester site, are encoded by the exon 18 and 24, respectively. Several possible regulatory signals such as a TPA-inducible enhancer core, an identifier sequence, purine-pyrimidine alternative stretches and viral enhancer core sequences were identified. Several genomic DNA clones which cross-hybridized with the α₂M cDNA probe were also identified. Sequence analysis showed that they possessed sequences identical to a part of the rat α₁-inhibitor III cDNA and that they had a strikingly similar exon organization to the α₂M gene.

Original language	English
Pages (from-to)	333-340
Number of pages	8
Journal	Gene
Volume	77
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(89)90081-4
Publication status	Published - 1989 Apr 30
Externally published	Yes

Keywords

Acute-phase protein
exon
gene family
intron
liver
nucleotide sequence
promoter
tissue inflammation

ASJC Scopus subject areas

Genetics

Access to Document

10.1016/0378-1119(89)90081-4

Cite this

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title = "Structure of the rat α2-macroglobulin-coding gene",

abstract = "Rat α2-macroglobulin (α2M) is an acute-phase protein i.e., produced upon tissue inflammation. Genomic DNA clones covering the entire sequence of the α2M gene were isolated and characterized by restriction mapping, Southern blotting and (partial) DNA sequencing. The rat α2M gene is approx. 50 kb in length and consists of 36 exons ranging in size from 21 to 229 bp. Two functional domains, a bait region and a thiol ester site, are encoded by the exon 18 and 24, respectively. Several possible regulatory signals such as a TPA-inducible enhancer core, an identifier sequence, purine-pyrimidine alternative stretches and viral enhancer core sequences were identified. Several genomic DNA clones which cross-hybridized with the α2M cDNA probe were also identified. Sequence analysis showed that they possessed sequences identical to a part of the rat α1-inhibitor III cDNA and that they had a strikingly similar exon organization to the α2M gene.",

keywords = "Acute-phase protein, exon, gene family, intron, liver, nucleotide sequence, promoter, tissue inflammation",

author = "Masahira Hattori and Kusakabe Shin-ichi and Ohgusu Hideko and Tsuchiya Yoshihiro and Ito Takashi and Sakaki Yoshiyuki",

year = "1989",

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AU - Hattori, Masahira

AU - Shin-ichi, Kusakabe

AU - Hideko, Ohgusu

AU - Yoshihiro, Tsuchiya

AU - Takashi, Ito

AU - Yoshiyuki, Sakaki

PY - 1989/4/30

Y1 - 1989/4/30

N2 - Rat α2-macroglobulin (α2M) is an acute-phase protein i.e., produced upon tissue inflammation. Genomic DNA clones covering the entire sequence of the α2M gene were isolated and characterized by restriction mapping, Southern blotting and (partial) DNA sequencing. The rat α2M gene is approx. 50 kb in length and consists of 36 exons ranging in size from 21 to 229 bp. Two functional domains, a bait region and a thiol ester site, are encoded by the exon 18 and 24, respectively. Several possible regulatory signals such as a TPA-inducible enhancer core, an identifier sequence, purine-pyrimidine alternative stretches and viral enhancer core sequences were identified. Several genomic DNA clones which cross-hybridized with the α2M cDNA probe were also identified. Sequence analysis showed that they possessed sequences identical to a part of the rat α1-inhibitor III cDNA and that they had a strikingly similar exon organization to the α2M gene.

AB - Rat α2-macroglobulin (α2M) is an acute-phase protein i.e., produced upon tissue inflammation. Genomic DNA clones covering the entire sequence of the α2M gene were isolated and characterized by restriction mapping, Southern blotting and (partial) DNA sequencing. The rat α2M gene is approx. 50 kb in length and consists of 36 exons ranging in size from 21 to 229 bp. Two functional domains, a bait region and a thiol ester site, are encoded by the exon 18 and 24, respectively. Several possible regulatory signals such as a TPA-inducible enhancer core, an identifier sequence, purine-pyrimidine alternative stretches and viral enhancer core sequences were identified. Several genomic DNA clones which cross-hybridized with the α2M cDNA probe were also identified. Sequence analysis showed that they possessed sequences identical to a part of the rat α1-inhibitor III cDNA and that they had a strikingly similar exon organization to the α2M gene.

KW - Acute-phase protein

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KW - gene family

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KW - nucleotide sequence

KW - promoter

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