TY - JOUR
T1 - Structures of genes encoding TATA box-binding proteins from trimeresurus gramineus and t. flavoviridis snakes
AU - Nakashima, Kin ichi
AU - Nobuhisa, Ikuo
AU - Deshimaru, Masanobu
AU - Ogawa, Tomohisa
AU - Shimohigashi, Yasuyuki
AU - Fukumaki, Yasuyuki
AU - Hattori, Masahira
AU - Sakaki, Yoshiyuki
AU - Hattori, Shosaku
AU - Ohno, Motonori
PY - 1995/1/23
Y1 - 1995/1/23
N2 - A cDNA encoding the Trimeresurus gramineus (Tg; green habu snake) TATA-box-binding protein (TgTBP) was cloned and sequenced. The cDNA encodes a 33-kDa protein with an extensive sequence similarity to those derived from other organisms, except for the N-terminal domain. Genes encoding TgTBP and Trimeresurus flavoviridis (Tf; habu snake) TBP (TgTBP) were isolated using a TgTBP cDNA and their nt sequences were determined. They are the first TBP genes entirely sequenced in higher animals. Both genes span over 15 kb and are constructed from eight exons and seven introns. Comparison of the loci of introns on the aligned amino-acid sequences of TBP from six organisms (Tg, Tf, mouse, Arabidopsis thaliana, Schizosaccharomyces pombe and Acanthamoeba castellanii) indicated that there are three highly conserved loci in the C-terminal domain.
AB - A cDNA encoding the Trimeresurus gramineus (Tg; green habu snake) TATA-box-binding protein (TgTBP) was cloned and sequenced. The cDNA encodes a 33-kDa protein with an extensive sequence similarity to those derived from other organisms, except for the N-terminal domain. Genes encoding TgTBP and Trimeresurus flavoviridis (Tf; habu snake) TBP (TgTBP) were isolated using a TgTBP cDNA and their nt sequences were determined. They are the first TBP genes entirely sequenced in higher animals. Both genes span over 15 kb and are constructed from eight exons and seven introns. Comparison of the loci of introns on the aligned amino-acid sequences of TBP from six organisms (Tg, Tf, mouse, Arabidopsis thaliana, Schizosaccharomyces pombe and Acanthamoeba castellanii) indicated that there are three highly conserved loci in the C-terminal domain.
KW - amino-acid sequence
KW - cDNA
KW - conserved C-terminal sequence
KW - glutamine-rich run
KW - reptile introns
KW - Transcription
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U2 - 10.1016/0378-1119(94)00681-H
DO - 10.1016/0378-1119(94)00681-H
M3 - Article
C2 - 7835702
AN - SCOPUS:0028917949
SN - 0378-1119
VL - 152
SP - 209
EP - 213
JO - Gene
JF - Gene
IS - 2
ER -