Study of the 'Raman spectroscopic signature of life' in mitochondria isolated from budding yeast

Liang da Chiu; Masahiro Ando; Hiro o. Hamaguchi

doi:10.1002/jrs.2425

Study of the 'Raman spectroscopic signature of life' in mitochondria isolated from budding yeast

Liang da Chiu, Masahiro Ando, Hiro o. Hamaguchi^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

The 'Raman spectroscopic signature of life' is a Raman band at 1602 cm^-1 that sharply reflects the metabolic activity of cell mitochondria. Here we report the study of thissignature in isolated yeast mitochondria. The existence and behaviour of the 1602 cm^-1 band in isolated mitochondria have been confirmed to be the same as in living yeast cells: the intensity of the band decreases with timewhen a respiration inhibitor, sodium azide, is added. The present study shows the significance of isolated mitochondria in elucidating the origin of this still unassigned Raman band.

Original language	English
Pages (from-to)	2-3
Number of pages	2
Journal	Journal of Raman Spectroscopy
Volume	41
Issue number	1
DOIs	https://doi.org/10.1002/jrs.2425
Publication status	Published - 2010 Jan
Externally published	Yes

Keywords

Budding yeast
Metabolic activity
Mitochondria
Raman spectroscopic signature of life
Respiration inhibition

ASJC Scopus subject areas

Materials Science(all)
Spectroscopy

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10.1002/jrs.2425

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abstract = "The 'Raman spectroscopic signature of life' is a Raman band at 1602 cm-1 that sharply reflects the metabolic activity of cell mitochondria. Here we report the study of thissignature in isolated yeast mitochondria. The existence and behaviour of the 1602 cm-1 band in isolated mitochondria have been confirmed to be the same as in living yeast cells: the intensity of the band decreases with timewhen a respiration inhibitor, sodium azide, is added. The present study shows the significance of isolated mitochondria in elucidating the origin of this still unassigned Raman band.",

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AB - The 'Raman spectroscopic signature of life' is a Raman band at 1602 cm-1 that sharply reflects the metabolic activity of cell mitochondria. Here we report the study of thissignature in isolated yeast mitochondria. The existence and behaviour of the 1602 cm-1 band in isolated mitochondria have been confirmed to be the same as in living yeast cells: the intensity of the band decreases with timewhen a respiration inhibitor, sodium azide, is added. The present study shows the significance of isolated mitochondria in elucidating the origin of this still unassigned Raman band.

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KW - Raman spectroscopic signature of life

KW - Respiration inhibition

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Study of the 'Raman spectroscopic signature of life' in mitochondria isolated from budding yeast

Abstract

Keywords

ASJC Scopus subject areas

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