Study on Some Factors Affecting the Precision of Luminescence Analyses

The effects of the shape of the reaction vessel, the sample volume and the volume and speed of the reagent injection on the reproducibility of the chemiluminescence (CL) analyses were investigated with a laboratory-built automated injection device and a computer system. The suitable selections of the vessel diameter as well as the injection volume are shown to be necessary for the precise measurements of samples of given volume. Under the optimal mixing conditions, the choice of signal processings such as peak height measurements or fixed-time integration did not affect the reproducibility and any of the methods used could give accurate CL values. These principles for the precise measurement represent useful guides for luminometer designing and experiments for rapid, precise analyses.