Substrate specificity of trna (adenine-1-)-methyltransferase from thermus thermophilus hb27

Norihiko Yamazaki, Hiroyuki Hori, Kiyoshi Ozawa, Shigeko Nakanishi, Takuya Ueda, Izumi Kumagai, Kimitsuna Watanabe, Kazuya Nishikawa

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9 Citations (Scopus)


Trna (adenine-1-)-methyItransferase was purified to homogeneity from an extreme thermophile, Thermus thermophilus HB27, by several steps of column chromatographies. The molecular weight of this enzyme was about 60,000 as analyzed by SDS polyacrylamide gel electrophoresis. Km for E. coli was 100 nm and that for the methyl group donor, S-adenosyl-l-methionine, was 7.8 μm. The substrate specificity of the enzyme was investigated by using T7 RNA polymerase transcripts and tRNA fragments obtained by partial digestion with RNases. The enzyme was able to transfer the methyl group to the 3′-half fragment of E. coli initiator tRNA, however, the extent of methylation was elevated by more than five times when the 5′-half fragment was added and annealed to the 3′-half. This indicates that the main recognition site of the enzyme is within the 3′-half region of tRNA molecule, while the tertiary interaction between the T-loop and the D-loop is very effective for the adequate methylation reaction.

Original languageEnglish
Pages (from-to)1128-1133
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Issue number6
Publication statusPublished - 1994 Jan
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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