Suppression of metHb formation in Hb-vesicles by glutathione

H. Yokohama; Y. Seino; J. E. Chung; H. Sakai; S. Takeoka; H. Nishide; E. Tsuchida

Suppression of metHb formation in Hb-vesicles by glutathione

H. Yokohama^*, Y. Seino, J. E. Chung, H. Sakai, S. Takeoka, H. Nishide, E. Tsuchida

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

To increase the amount of oxygen transported by Hb-vesicles, it is important to suppress metHb formation. α-Tocopherol decreased lipid peroxidation in Hb-vesicles composed of EYL, whereas the Hb-vesicles composed of saturated phospholipids, such as DPPC, showed less metHb formation. To suppress metHb formation in a highly purified Hb solution, glutathione (GSH) was studied as a reductant. GSH is oxidized not only by metHb reduction but also by O₂ to generate active oxygen species, which react with Hb to form metHb. Effective nonenzymatic metHb reduction was observed at a high [Hb] because of the acceleration of metHb reduction, and at lower partial pressure of O₂ (pO₂) because of suppression of GSH oxidation. Hb-vesicles encapsulating 40 g/dl Hb with pyridoxal 5'-phosphate showed high oxygen transporting efficiency. The rate of metHb formation at a low pO₂ in the blood stream was decreased by GSH.

Original language	English
Pages (from-to)	868-871
Number of pages	4
Journal	Japanese Journal of Artificial Organs
Volume	23
Issue number	3
Publication status	Published - 1994 Jan 1

Keywords

Hb-vesicles
glutathione
lipid peroxidation
metHb reduction
α-tocopherol

ASJC Scopus subject areas

Biophysics

Cite this

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title = "Suppression of metHb formation in Hb-vesicles by glutathione",

abstract = "To increase the amount of oxygen transported by Hb-vesicles, it is important to suppress metHb formation. α-Tocopherol decreased lipid peroxidation in Hb-vesicles composed of EYL, whereas the Hb-vesicles composed of saturated phospholipids, such as DPPC, showed less metHb formation. To suppress metHb formation in a highly purified Hb solution, glutathione (GSH) was studied as a reductant. GSH is oxidized not only by metHb reduction but also by O2 to generate active oxygen species, which react with Hb to form metHb. Effective nonenzymatic metHb reduction was observed at a high [Hb] because of the acceleration of metHb reduction, and at lower partial pressure of O2 (pO2) because of suppression of GSH oxidation. Hb-vesicles encapsulating 40 g/dl Hb with pyridoxal 5'-phosphate showed high oxygen transporting efficiency. The rate of metHb formation at a low pO2 in the blood stream was decreased by GSH.",

keywords = "Hb-vesicles, glutathione, lipid peroxidation, metHb reduction, α-tocopherol",

author = "H. Yokohama and Y. Seino and Chung, {J. E.} and H. Sakai and S. Takeoka and H. Nishide and E. Tsuchida",

year = "1994",

month = jan,

day = "1",

language = "English",

volume = "23",

pages = "868--871",

journal = "Japanese Journal of Artificial Organs",

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TY - JOUR

T1 - Suppression of metHb formation in Hb-vesicles by glutathione

AU - Yokohama, H.

AU - Seino, Y.

AU - Chung, J. E.

AU - Sakai, H.

AU - Takeoka, S.

AU - Nishide, H.

AU - Tsuchida, E.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - To increase the amount of oxygen transported by Hb-vesicles, it is important to suppress metHb formation. α-Tocopherol decreased lipid peroxidation in Hb-vesicles composed of EYL, whereas the Hb-vesicles composed of saturated phospholipids, such as DPPC, showed less metHb formation. To suppress metHb formation in a highly purified Hb solution, glutathione (GSH) was studied as a reductant. GSH is oxidized not only by metHb reduction but also by O2 to generate active oxygen species, which react with Hb to form metHb. Effective nonenzymatic metHb reduction was observed at a high [Hb] because of the acceleration of metHb reduction, and at lower partial pressure of O2 (pO2) because of suppression of GSH oxidation. Hb-vesicles encapsulating 40 g/dl Hb with pyridoxal 5'-phosphate showed high oxygen transporting efficiency. The rate of metHb formation at a low pO2 in the blood stream was decreased by GSH.

AB - To increase the amount of oxygen transported by Hb-vesicles, it is important to suppress metHb formation. α-Tocopherol decreased lipid peroxidation in Hb-vesicles composed of EYL, whereas the Hb-vesicles composed of saturated phospholipids, such as DPPC, showed less metHb formation. To suppress metHb formation in a highly purified Hb solution, glutathione (GSH) was studied as a reductant. GSH is oxidized not only by metHb reduction but also by O2 to generate active oxygen species, which react with Hb to form metHb. Effective nonenzymatic metHb reduction was observed at a high [Hb] because of the acceleration of metHb reduction, and at lower partial pressure of O2 (pO2) because of suppression of GSH oxidation. Hb-vesicles encapsulating 40 g/dl Hb with pyridoxal 5'-phosphate showed high oxygen transporting efficiency. The rate of metHb formation at a low pO2 in the blood stream was decreased by GSH.

KW - Hb-vesicles

KW - glutathione

KW - lipid peroxidation

KW - metHb reduction

KW - α-tocopherol

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M3 - Article

AN - SCOPUS:0028228397

SN - 0300-0818

VL - 23

SP - 868

EP - 871

JO - Japanese Journal of Artificial Organs

JF - Japanese Journal of Artificial Organs

IS - 3

ER -

Suppression of metHb formation in Hb-vesicles by glutathione

Abstract

Keywords

ASJC Scopus subject areas

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