Surface charge density estimation of vigna mungo protoplasts using a fluorescent dye, 9-aminoacridine

Kotaro Oka; Hiroko Ikeshima; Hideo Ishikawa; Eiji Ohta; Makoto Sakata

Surface charge density estimation of vigna mungo protoplasts using a fluorescent dye, 9-aminoacridine

Kotaro Oka^*, Hiroko Ikeshima, Hideo Ishikawa, Eiji Ohta, Makoto Sakata

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

We showed that the surface charge density of protoplasts can be estimated by the 9-aminoacridine method. The estimated surface charge density of the protoplasts isolated from elongating regions of Vigna mungo root was - 39 ± 8 mC/m². The negative surface charge density increased when protoplasts were treated with glutaraldehyde or when EDTA was added to the protoplast suspension medium. These results support the validity of our estimation of the surface charge density of protoplasts by the 9-aminoacridine method. The concentration of amino groups at the surface of the protoplasts was estimated to be 34 mC/m².

Original language	English
Pages (from-to)	771-775
Number of pages	5
Journal	Plant and Cell Physiology
Volume	29
Issue number	5
Publication status	Published - 1988 Jul
Externally published	Yes

Keywords

9-Aminoacridine
EDTA
Glutaraldehyde
Surface charge density
Vigna mungo (Protoplasts)

ASJC Scopus subject areas

Statistics, Probability and Uncertainty
Applied Mathematics
Ecology
Cell Biology
Physiology
Plant Science

Cite this

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title = "Surface charge density estimation of vigna mungo protoplasts using a fluorescent dye, 9-aminoacridine",

abstract = "We showed that the surface charge density of protoplasts can be estimated by the 9-aminoacridine method. The estimated surface charge density of the protoplasts isolated from elongating regions of Vigna mungo root was - 39 ± 8 mC/m2. The negative surface charge density increased when protoplasts were treated with glutaraldehyde or when EDTA was added to the protoplast suspension medium. These results support the validity of our estimation of the surface charge density of protoplasts by the 9-aminoacridine method. The concentration of amino groups at the surface of the protoplasts was estimated to be 34 mC/m2.",

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year = "1988",

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T1 - Surface charge density estimation of vigna mungo protoplasts using a fluorescent dye, 9-aminoacridine

AU - Oka, Kotaro

AU - Ikeshima, Hiroko

AU - Ishikawa, Hideo

AU - Ohta, Eiji

AU - Sakata, Makoto

PY - 1988/7

Y1 - 1988/7

N2 - We showed that the surface charge density of protoplasts can be estimated by the 9-aminoacridine method. The estimated surface charge density of the protoplasts isolated from elongating regions of Vigna mungo root was - 39 ± 8 mC/m2. The negative surface charge density increased when protoplasts were treated with glutaraldehyde or when EDTA was added to the protoplast suspension medium. These results support the validity of our estimation of the surface charge density of protoplasts by the 9-aminoacridine method. The concentration of amino groups at the surface of the protoplasts was estimated to be 34 mC/m2.

AB - We showed that the surface charge density of protoplasts can be estimated by the 9-aminoacridine method. The estimated surface charge density of the protoplasts isolated from elongating regions of Vigna mungo root was - 39 ± 8 mC/m2. The negative surface charge density increased when protoplasts were treated with glutaraldehyde or when EDTA was added to the protoplast suspension medium. These results support the validity of our estimation of the surface charge density of protoplasts by the 9-aminoacridine method. The concentration of amino groups at the surface of the protoplasts was estimated to be 34 mC/m2.

KW - 9-Aminoacridine

KW - EDTA

KW - Glutaraldehyde

KW - Surface charge density

KW - Vigna mungo (Protoplasts)

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Surface charge density estimation of vigna mungo protoplasts using a fluorescent dye, 9-aminoacridine

Abstract

Keywords

ASJC Scopus subject areas

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