Synaptic function and neuropathological disease revealed by quantum dot-single-particle tracking

Hiroko Bannai*, Takafumi Inoue, Matsumi Hirose, Fumihiro Niwa, Katsuhiko Mikoshiba

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)


Quantum dot-single-particle tracking (QD-SPT) is a super-resolution imaging technique that uses semiconductor nanocrystal quantum dots as fluorescent probes and is a powerful tool for analyzing protein and lipid behavior in the plasma membrane. Recent QD-SPT experiments have provided critical insight into the mechanism and physiological relevance of membrane self-organization in neurons and astrocytes in the brain. The mobility of some membrane molecules may become abnormal in cellular models of epilepsy and Alzheimer’s disease. Based on these findings, we propose that the behavior of membrane molecules reflects the condition of neurons in pathological disease states. In this chapter, we describe the latest, simple QD-SPT technique, which is feasible with epifluorescence microscopy and dissociated cell cultures.

Original languageEnglish
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Number of pages25
Publication statusPublished - 2020

Publication series

ISSN (Print)0893-2336
ISSN (Electronic)1940-6045


  • Astrocyte
  • Dissociated culture
  • Ion channel
  • Lateral diffusion
  • Lipids
  • Membrane molecules
  • Neuron
  • Quantum dot
  • Receptor
  • Single-particle tracking
  • Synapse

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Psychiatry and Mental health


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