Synthesis, characterization and cytotoxicity evaluation on zinc doped hydroxyapatite in collagen matrix

C. L. Popa; C. M. Bartha; M. Albu; R. Guegan; M. Motelica-Heino; M. C. Chifiriuc; C. Bleotu; M. L. Badea; S. Antohe

Synthesis, characterization and cytotoxicity evaluation on zinc doped hydroxyapatite in collagen matrix

C. L. Popa, C. M. Bartha, M. Albu, R. Guegan, M. Motelica-Heino, M. C. Chifiriuc, C. Bleotu, M. L. Badea, S. Antohe^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

The goal of this study was to obtain at low temperature a functional nano-composite with characteristics similar to the natural bone by using a cost effective method. The structure and morphology of collagen coated zinc doped hydroxyapatite bio-composites (Zn:HAp-CBc) were examined by X-Ray diffraction (XRD) and Scanning Electron Microscopy (SEM). XRD analysis revealed that the unique hexagonal Ca₁₀(PO₄)₆(OH)₂ in P63m space group was observed in the obtained nanocomposites Zn:HAp-CBc. The cytotoxicity of the Zn:HAp-CBc was studied on HeLa cell lines. Cell cycle distribution after treatment was examined by flow cytometry analysis. Our preliminary in vitro studies revealed that the obtained composites based on Zn doped HAp embedded in collagen matrix have excellent biocompatibility and support their further characterization by in vivo approaches and development as a biomaterial used in bone regeneration.

Original language	English
Pages (from-to)	681-691
Number of pages	11
Journal	Digest Journal of Nanomaterials and Biostructures
Volume	10
Issue number	2
Publication status	Published - 2015
Externally published	Yes

Keywords

Bio-composite
Collagen
Hydroxyapatite
Zinc

ASJC Scopus subject areas

Structural Biology
Atomic and Molecular Physics, and Optics
Biomedical Engineering
Materials Science(all)
Condensed Matter Physics
Physical and Theoretical Chemistry

Cite this

@article{d5c75e0353094cd995ceed936c6e7081,

title = "Synthesis, characterization and cytotoxicity evaluation on zinc doped hydroxyapatite in collagen matrix",

abstract = "The goal of this study was to obtain at low temperature a functional nano-composite with characteristics similar to the natural bone by using a cost effective method. The structure and morphology of collagen coated zinc doped hydroxyapatite bio-composites (Zn:HAp-CBc) were examined by X-Ray diffraction (XRD) and Scanning Electron Microscopy (SEM). XRD analysis revealed that the unique hexagonal Ca10(PO4)6(OH)2 in P63m space group was observed in the obtained nanocomposites Zn:HAp-CBc. The cytotoxicity of the Zn:HAp-CBc was studied on HeLa cell lines. Cell cycle distribution after treatment was examined by flow cytometry analysis. Our preliminary in vitro studies revealed that the obtained composites based on Zn doped HAp embedded in collagen matrix have excellent biocompatibility and support their further characterization by in vivo approaches and development as a biomaterial used in bone regeneration.",

keywords = "Bio-composite, Collagen, Hydroxyapatite, Zinc",

author = "Popa, {C. L.} and Bartha, {C. M.} and M. Albu and R. Guegan and M. Motelica-Heino and Chifiriuc, {M. C.} and C. Bleotu and Badea, {M. L.} and S. Antohe",

year = "2015",

language = "English",

volume = "10",

pages = "681--691",

journal = "Digest Journal of Nanomaterials and Biostructures",

issn = "1842-3582",

publisher = "National Institute R and D of Materials Physics",

number = "2",

}

TY - JOUR

T1 - Synthesis, characterization and cytotoxicity evaluation on zinc doped hydroxyapatite in collagen matrix

AU - Popa, C. L.

AU - Bartha, C. M.

AU - Albu, M.

AU - Guegan, R.

AU - Motelica-Heino, M.

AU - Chifiriuc, M. C.

AU - Bleotu, C.

AU - Badea, M. L.

AU - Antohe, S.

PY - 2015

Y1 - 2015

N2 - The goal of this study was to obtain at low temperature a functional nano-composite with characteristics similar to the natural bone by using a cost effective method. The structure and morphology of collagen coated zinc doped hydroxyapatite bio-composites (Zn:HAp-CBc) were examined by X-Ray diffraction (XRD) and Scanning Electron Microscopy (SEM). XRD analysis revealed that the unique hexagonal Ca10(PO4)6(OH)2 in P63m space group was observed in the obtained nanocomposites Zn:HAp-CBc. The cytotoxicity of the Zn:HAp-CBc was studied on HeLa cell lines. Cell cycle distribution after treatment was examined by flow cytometry analysis. Our preliminary in vitro studies revealed that the obtained composites based on Zn doped HAp embedded in collagen matrix have excellent biocompatibility and support their further characterization by in vivo approaches and development as a biomaterial used in bone regeneration.

AB - The goal of this study was to obtain at low temperature a functional nano-composite with characteristics similar to the natural bone by using a cost effective method. The structure and morphology of collagen coated zinc doped hydroxyapatite bio-composites (Zn:HAp-CBc) were examined by X-Ray diffraction (XRD) and Scanning Electron Microscopy (SEM). XRD analysis revealed that the unique hexagonal Ca10(PO4)6(OH)2 in P63m space group was observed in the obtained nanocomposites Zn:HAp-CBc. The cytotoxicity of the Zn:HAp-CBc was studied on HeLa cell lines. Cell cycle distribution after treatment was examined by flow cytometry analysis. Our preliminary in vitro studies revealed that the obtained composites based on Zn doped HAp embedded in collagen matrix have excellent biocompatibility and support their further characterization by in vivo approaches and development as a biomaterial used in bone regeneration.

KW - Bio-composite

KW - Collagen

KW - Hydroxyapatite

KW - Zinc

UR - http://www.scopus.com/inward/record.url?scp=84935857593&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84935857593&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84935857593

SN - 1842-3582

VL - 10

SP - 681

EP - 691

JO - Digest Journal of Nanomaterials and Biostructures

JF - Digest Journal of Nanomaterials and Biostructures

IS - 2

ER -

Synthesis, characterization and cytotoxicity evaluation on zinc doped hydroxyapatite in collagen matrix

Abstract

Keywords

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this