Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast

Ngang Heok Tang; Naoyuki Okada; Chii Shyang Fong; Kunio Arai; Masamitsu Sato; Takashi Toda

doi:10.1016/j.febslet.2014.06.027

Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast

Ngang Heok Tang, Naoyuki Okada, Chii Shyang Fong, Kunio Arai, Masamitsu Sato, Takashi Toda^*

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

The conserved TACC protein family localises to the centrosome (the spindle pole body, SPB in fungi) and mitotic spindles, thereby playing a crucial role in bipolar spindle assembly. However, it remains elusive how TACC proteins are recruited to the centrosome/SPB. Here, using fission yeast Alp7/TACC, we have determined clustered five amino acid residues within the TACC domain required for SPB localisation. Critically, these sequences are essential for the functions of Alp7, including proper spindle formation and mitotic progression. Moreover, we have identified pericentrin-like Pcp1 as a loading factor to the mitotic SPB, although Pcp1 is not a sole platform.

Original language	English
Pages (from-to)	2814-2821
Number of pages	8
Journal	FEBS Letters
Volume	588
Issue number	17
DOIs	https://doi.org/10.1016/j.febslet.2014.06.027
Publication status	Published - 2014 Aug 25

Keywords

Centrosome
Fission yeast
Pericentrin family
Spindle microtubule
Spindle pole body
TACC

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast",

abstract = "The conserved TACC protein family localises to the centrosome (the spindle pole body, SPB in fungi) and mitotic spindles, thereby playing a crucial role in bipolar spindle assembly. However, it remains elusive how TACC proteins are recruited to the centrosome/SPB. Here, using fission yeast Alp7/TACC, we have determined clustered five amino acid residues within the TACC domain required for SPB localisation. Critically, these sequences are essential for the functions of Alp7, including proper spindle formation and mitotic progression. Moreover, we have identified pericentrin-like Pcp1 as a loading factor to the mitotic SPB, although Pcp1 is not a sole platform.",

keywords = "Centrosome, Fission yeast, Pericentrin family, Spindle microtubule, Spindle pole body, TACC",

author = "Tang, {Ngang Heok} and Naoyuki Okada and Fong, {Chii Shyang} and Kunio Arai and Masamitsu Sato and Takashi Toda",

note = "Funding Information: We thank Risa Mori for critical reading of the manuscript and useful suggestions, and Masayuki Yamamoto for generous support during the course of this study. K.A. and N.O. were research fellows of the Japan Society for the Promotion of Science (JSPS). This study was supported by JSPS KAKENHI : Grants-in-Aid for Young Scientists (A) (Grant Number 21687015 ), for Scientific Research (B) ( 25291041 ), the Sumitomo Foundation, the Naito Foundation and the Kishimoto Grant from the Senri Life Science Foundation (to M.S.) and Cancer Research UK (T.T.). ",

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AU - Tang, Ngang Heok

AU - Okada, Naoyuki

AU - Fong, Chii Shyang

AU - Arai, Kunio

AU - Sato, Masamitsu

AU - Toda, Takashi

N1 - Funding Information: We thank Risa Mori for critical reading of the manuscript and useful suggestions, and Masayuki Yamamoto for generous support during the course of this study. K.A. and N.O. were research fellows of the Japan Society for the Promotion of Science (JSPS). This study was supported by JSPS KAKENHI : Grants-in-Aid for Young Scientists (A) (Grant Number 21687015 ), for Scientific Research (B) ( 25291041 ), the Sumitomo Foundation, the Naito Foundation and the Kishimoto Grant from the Senri Life Science Foundation (to M.S.) and Cancer Research UK (T.T.).

PY - 2014/8/25

Y1 - 2014/8/25

N2 - The conserved TACC protein family localises to the centrosome (the spindle pole body, SPB in fungi) and mitotic spindles, thereby playing a crucial role in bipolar spindle assembly. However, it remains elusive how TACC proteins are recruited to the centrosome/SPB. Here, using fission yeast Alp7/TACC, we have determined clustered five amino acid residues within the TACC domain required for SPB localisation. Critically, these sequences are essential for the functions of Alp7, including proper spindle formation and mitotic progression. Moreover, we have identified pericentrin-like Pcp1 as a loading factor to the mitotic SPB, although Pcp1 is not a sole platform.

AB - The conserved TACC protein family localises to the centrosome (the spindle pole body, SPB in fungi) and mitotic spindles, thereby playing a crucial role in bipolar spindle assembly. However, it remains elusive how TACC proteins are recruited to the centrosome/SPB. Here, using fission yeast Alp7/TACC, we have determined clustered five amino acid residues within the TACC domain required for SPB localisation. Critically, these sequences are essential for the functions of Alp7, including proper spindle formation and mitotic progression. Moreover, we have identified pericentrin-like Pcp1 as a loading factor to the mitotic SPB, although Pcp1 is not a sole platform.

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Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast

Abstract

Keywords

ASJC Scopus subject areas

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