Temperature dependence for anammox bacteria enriched from freshwater sediments

Toshifumi Osaka, Yuya Kimura, Yosuke Otsubo, Yuichi Suwa, Satoshi Tsuneda*, Kazuichi Isaka

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)


The anoxic ammonium oxidation (anammox) process has been regarded as an attractive alternative process to treat wastewater containing high ammonium concentrations. By the implementation of anammox process at moderately low temperatures (<25°C), the anammox process will be applied to more various industrial wastewater treatments. In this study, we established enrichment cultures of anammox bacteria from freshwater sediments by using an up-flow column reactor equipped with porous polyester nonwoven fabric at moderately low temperatures. Their nitrogen conversion rates reached 0.07-0.26kg-N/m3/d. Phylogenetic analysis based on 16S rRNA gene from enrichment cultures revealed the presence of various anammox bacteria affiliated with unknown anammox bacteria as well as known anammox candidates, i.e., Candidatus Kuenenia stuttgartiensis and Candidatus Brocadia fulgida, Candidatus Scalindua wagneri. Anammox bacterial populations were influenced by enrichment conditions, i.e., seed sediments and temperature.

Original languageEnglish
Pages (from-to)429-434
Number of pages6
JournalJournal of Bioscience and Bioengineering
Issue number4
Publication statusPublished - 2012 Oct


  • 16S rRNA phylogenic analysis
  • Anammox
  • Biological nitrogen cycle
  • Freshwater environment
  • Moderately low temperature

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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