TY - JOUR
T1 - Temperature-Responsive Liposome-Linked Immunosorbent Assay for the Rapid Detection of SARS-CoV-2 Using Immunoliposomes
AU - Hu, Runkai
AU - Hotta, Morihiro
AU - Maruyama, Taro
AU - Fujisawa, Mizuki
AU - Sou, Keitaro
AU - Takeoka, Shinji
N1 - Funding Information:
This work was partly supported by AMED under grant number JP21lm0203004. This work was the result of using research equipment (JEM-1011: Material Characterization Central Laboratory) shared in MEXT Project for promoting public utilization of advanced research infrastructure (Program for supporting construction of core facilities)––grant number JPMXS0440500022. We acknowledge Dr. Kentaro Miyoshi (Materials Characterization Central Laboratory, Waseda University) for the technical support with the TEM observation. We thank Edanz ( https://jp.edanz.com/ac ) for editing a draft of this manuscript.
Publisher Copyright:
© 2022 American Chemical Society. All rights reserved.
PY - 2022/8/2
Y1 - 2022/8/2
N2 - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is the etiological agent of coronavirus disease 2019 (COVID-19), has infected more than 340 million people since the outbreak of the pandemic in 2019, resulting in approximately 55 million deaths. The rapid and effective diagnosis of COVID-19 patients is vital to prevent the spread of the disease. In a previous study, we reported a novel temperature-responsive liposome-linked immunosorbent assay (TLip-LISA) using biotinylated-TLip that exhibited high detection sensitivity for the prostate-specific antigen. Herein, we used immunoglobulin-TLip (IgG-TLip), in which the antibodies were directly conjugated to the liposomal surface to simplify pretreatment procedures and reduce the detection time for SARS-CoV-2. The results indicated that TLip-LISA could detect the recombinant nucleocapsid protein and the nucleocapsid protein in inactivated virus with 20 min incubation time in total, and the limit of detection was calculated to be 2.2 and 1.0 pg/mL, respectively. Therefore, TLip-LISA has high potential to be used in clinic for rapid diagnosis and disease control.
AB - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is the etiological agent of coronavirus disease 2019 (COVID-19), has infected more than 340 million people since the outbreak of the pandemic in 2019, resulting in approximately 55 million deaths. The rapid and effective diagnosis of COVID-19 patients is vital to prevent the spread of the disease. In a previous study, we reported a novel temperature-responsive liposome-linked immunosorbent assay (TLip-LISA) using biotinylated-TLip that exhibited high detection sensitivity for the prostate-specific antigen. Herein, we used immunoglobulin-TLip (IgG-TLip), in which the antibodies were directly conjugated to the liposomal surface to simplify pretreatment procedures and reduce the detection time for SARS-CoV-2. The results indicated that TLip-LISA could detect the recombinant nucleocapsid protein and the nucleocapsid protein in inactivated virus with 20 min incubation time in total, and the limit of detection was calculated to be 2.2 and 1.0 pg/mL, respectively. Therefore, TLip-LISA has high potential to be used in clinic for rapid diagnosis and disease control.
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U2 - 10.1021/acsomega.2c03597
DO - 10.1021/acsomega.2c03597
M3 - Article
AN - SCOPUS:85135892782
SN - 2470-1343
VL - 7
SP - 26936
EP - 26944
JO - ACS Omega
JF - ACS Omega
IS - 30
ER -