TY - JOUR
T1 - Tepidicaulis Marinus gen. nov., sp. nov., a marine bacterium that reduces nitrate to nitrous oxide under strictly microaerobic conditions
AU - Takeuchi, Mio
AU - Yamagishi, Takao
AU - Kamagata, Yoichi
AU - Oshima, Kenshiro
AU - Hattori, Masahira
AU - Katayama, Taiki
AU - Hanada, Satoshi
AU - Tamaki, Hideyuki
AU - Marumo, Katsumi
AU - Maeda, Hiroto
AU - Nedachi, Munetomo
AU - Iwasaki, Wataru
AU - Suwa, Yuichi
AU - Sakata, Susumu
PY - 2015/6/1
Y1 - 2015/6/1
N2 - A moderately thermophilic, aerobic, stalked bacterium (strain MA2T) was isolated from marine sediments in Kagoshima Bay, Japan. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain MA2Twas most closely related to the genera Rhodobium, Parvibaculum, and Rhodoligotrophos (92–93% similarity) within the class Alphaproteobacteria. Strain MA2T was a Gram-stain-negative and stalked dimorphic bacteria. The temperature range for growth was 16– 48 ºC (optimum growth at 42 ºC). This strain required yeast extract and NaCl (>1%, w/v) for growth, tolerated up to 11% (w/v) NaCl, and was capable of utilizing various carbon sources. The major cellular fatty acid and major respiratory quinone were C18: 1ω7c and ubiquinone-10, respectively. The DNA G+C content was 60.7 mol%. Strain MA2Tperformed denitrification and produced N2O from nitrate under strictly microaerobic conditions. Strain MA2Tpossessed periplasmic nitrate reductase (Nap) genes but not membrane-bound nitrate reductase (Nar) genes. On the basis of this morphological, physiological, biochemical and genetic information a novel genus and species, Tepidicaulis marinus gen. nov., sp. nov., are proposed, with MA2T(=NBRC 109643T=DSM 27167T) as the type strain of the species.
AB - A moderately thermophilic, aerobic, stalked bacterium (strain MA2T) was isolated from marine sediments in Kagoshima Bay, Japan. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain MA2Twas most closely related to the genera Rhodobium, Parvibaculum, and Rhodoligotrophos (92–93% similarity) within the class Alphaproteobacteria. Strain MA2T was a Gram-stain-negative and stalked dimorphic bacteria. The temperature range for growth was 16– 48 ºC (optimum growth at 42 ºC). This strain required yeast extract and NaCl (>1%, w/v) for growth, tolerated up to 11% (w/v) NaCl, and was capable of utilizing various carbon sources. The major cellular fatty acid and major respiratory quinone were C18: 1ω7c and ubiquinone-10, respectively. The DNA G+C content was 60.7 mol%. Strain MA2Tperformed denitrification and produced N2O from nitrate under strictly microaerobic conditions. Strain MA2Tpossessed periplasmic nitrate reductase (Nap) genes but not membrane-bound nitrate reductase (Nar) genes. On the basis of this morphological, physiological, biochemical and genetic information a novel genus and species, Tepidicaulis marinus gen. nov., sp. nov., are proposed, with MA2T(=NBRC 109643T=DSM 27167T) as the type strain of the species.
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U2 - 10.1099/ijs.0.000167
DO - 10.1099/ijs.0.000167
M3 - Article
C2 - 25740933
AN - SCOPUS:84938093608
SN - 1466-5026
VL - 65
SP - 1749
EP - 1754
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 6
ER -
