TY - JOUR
T1 - The association of elevated reactive oxygen species levels from neutrophils with low-grade inflammation in the elderly
AU - Ogawa, Kishiko
AU - Suzuki, Katsuhiko
AU - Mitsuharu, Okutsu
AU - Yamazaki, Kyoko
AU - Shinkai, Shoji
N1 - Funding Information:
We thank a former colleague Takahiro Yamazaki in Waseda University for supporting the experiments and a colleague Hidenori Amano in Tokyo Metropolitan Institute of Gerontology for supporting the statistic analysis. This study was supported by a Grant-in-Aid for the Young Scientist (A) and the Academic Frontier Project of the Ministry of Education, Culture, Sports, Science and Technology & Senior Research Partnership Project in the Faculty of Human Science, Waseda University.
PY - 2008/10/24
Y1 - 2008/10/24
N2 - Background: Reactive oxygen species (ROS), including free radicals, oxygen ions, and peroxides, are implicated in cell damage. The objective of this study was to investigate whether the spontaneous production of ROS from neutrophils changes with age and is associated with the conventional inflammatory markers. Results: Thirty-seven elderly subjects (median age, 87, range 70-95 years) and 22 young subjects (median age, 26, range 21-37 years) participated in this study. Circulating levels of C-reactive protein, serum amyloid A, tumor necrosis factor-α, interleukin (IL)-1, IL-6, IL-8, monocyte chemotactic protein-1, and heat shock protein (HSP)70 were measured with enzyme-linked immunosorbent assays. The N-formyl-methionyl-leucyl-phenylalanine and lipopolysaccharide-stimulated ROS of neutrophils were quantified by flow cytometry. Both spontaneous ROS production and circulating levels of inflammatory markers were higher in the elderly group than in the younger group. In addition, spontaneous ROS production by neutrophils was negatively associated with HSP70 in plasma. We could not find the association between spontaneous ROS production by neutrophils and the other inflammatory markers including cytokines. Conclusion: The results suggest that spontaneous ROS production from neutrophils may increase with age and represent the different aspect of age-associated immune dysregulation.
AB - Background: Reactive oxygen species (ROS), including free radicals, oxygen ions, and peroxides, are implicated in cell damage. The objective of this study was to investigate whether the spontaneous production of ROS from neutrophils changes with age and is associated with the conventional inflammatory markers. Results: Thirty-seven elderly subjects (median age, 87, range 70-95 years) and 22 young subjects (median age, 26, range 21-37 years) participated in this study. Circulating levels of C-reactive protein, serum amyloid A, tumor necrosis factor-α, interleukin (IL)-1, IL-6, IL-8, monocyte chemotactic protein-1, and heat shock protein (HSP)70 were measured with enzyme-linked immunosorbent assays. The N-formyl-methionyl-leucyl-phenylalanine and lipopolysaccharide-stimulated ROS of neutrophils were quantified by flow cytometry. Both spontaneous ROS production and circulating levels of inflammatory markers were higher in the elderly group than in the younger group. In addition, spontaneous ROS production by neutrophils was negatively associated with HSP70 in plasma. We could not find the association between spontaneous ROS production by neutrophils and the other inflammatory markers including cytokines. Conclusion: The results suggest that spontaneous ROS production from neutrophils may increase with age and represent the different aspect of age-associated immune dysregulation.
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U2 - 10.1186/1742-4933-5-13
DO - 10.1186/1742-4933-5-13
M3 - Article
C2 - 18950479
AN - SCOPUS:56349156642
SN - 1742-4933
VL - 5
JO - Immunity and Ageing
JF - Immunity and Ageing
M1 - 13
ER -