@article{071352b44b6c4582ba0f3f2621c36c38,
title = "The cell competition-based high-throughput screening identifies small compounds that promote the elimination of RasV12-transformed cells from epithelia",
abstract = "Recent studies have revealed that cell competition can occur between normal and transformed epithelial cells; normal epithelial cells recognize the presence of the neighboring transformed cells and actively eliminate them from epithelial tissues. Here, we have established a brand-new high-throughput screening platform that targets cell competition. By using this platform, we have identified Rebeccamycin as a hit compound that specifically promotes elimination of RasV12-transformed cells from the epithelium, though after longer treatment it shows substantial cytotoxic effect against normal epithelial cells. Among several Rebeccamycin-derivative compounds, we have found that VC1-8 has least cytotoxicity against normal cells but shows the comparable effect on the elimination of transformed cells. This cell competition-promoting activity of VC1-8 is observed both in vitro and ex vivo. These data demonstrate that the cell competition-based screening is a promising tool for the establishment of a novel type of cancer preventive medicine.",
author = "Hajime Yamauchi and Takanori Matsumaru and Tomoko Morita and Susumu Ishikawa and Katsumi Maenaka and Ichigaku Takigawa and Kentaro Semba and Shunsuke Kon and Yasuyuki Fujita",
note = "Funding Information: We thank Open Innovation Center for Drug Discovery (Tokyo University) for providing the library of small chemical compounds. We also thank Drs. S. Yokoshima and S. Ichikawa for discussion. This work was supported by Platform for Drug Discovery, Informatics, and Structural Life Science from the Ministry of Education, Culture, Sports, Science and Technology, and The Matching Program for Innovations in Future Drug Discovery and Medical Care, Japan. YF is supported by Funding Program for Grant-in-Aid for Scientific Research on Innovative Areas, Grant-in-Aid for Scientific Research (A), and Strategic Japanese-Swiss Cooperative Program. YF is also supported by the Takeda Science Foundation and the Uehara Memorial Foundation. HY is supported by Grant-in-Aid for Young Scientists (B) and the Yasuda Medical Foundation.",
year = "2015",
month = oct,
day = "20",
doi = "10.1038/srep15336",
language = "English",
volume = "5",
journal = "Scientific reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
}