The combined effects of magnesium oxide and inulin on intestinal microbiota and cecal short-chain fatty acids

Kanako Omori, Hiroki Miyakawa, Aya Watanabe, Yuki Nakayama, Yijin Lyu, Natsumi Ichikawa, Hiroyuki Sasaki, Shigenobu Shibata*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Constipation is a common condition that occurs in many people worldwide. While magnesium oxide (MgO) is often used as the first-line drug for chronic constipation in Japan, dietary fiber intake is also recommended. Dietary fiber is fermented by microbiota to produce short-chain fatty acids (SCFAs). SCFAs are involved in regulating systemic physiological functions and circadian rhythm. We examined the effect of combining MgO and the water-soluble dietary fiber, inulin, on cecal SCFA concentration and microbiota in mice. We also examined the MgO administration timing effect on cecal SCFAs. The cecal SCFA concentrations were measured by gas chromatography, and the microbiota was determined using next-generation sequencing. Inulin intake decreased cecal pH and increased cecal SCFA concentrations while combining MgO increased the cecal pH lowered by inulin and decreased the cecal SCFA concentrations elevated by inulin. When inulin and MgO were combined, significant changes in the microbiota composition were observed compared with inulin alone. The MgO effect on the cecal acetic acid concentration was less when administered at ZT12 than at ZT0. In conclusion, this study suggests that MgO affects cecal SCFA and microbiota during inulin feeding, and the effect on acetic acid concentration is time-dependent.

Original languageEnglish
Article number152
Pages (from-to)1-20
Number of pages20
Issue number1
Publication statusPublished - 2021 Jan


  • Administration timing
  • Intestinal flora
  • Laxative
  • MgO
  • SCFA
  • Water-soluble dietary fiber

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics


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