TY - JOUR
T1 - The efficacy of basic fibroblast growth factor-loaded poly(lactic-co-glycolic acid) nanosheet for mouse wound healing
AU - Aoki, Shimpo
AU - Fujii, Mao
AU - Fujie, Toshinori
AU - Nishiwaki, Keisuke
AU - Miyazaki, Hiromi
AU - Saitoh, Daizoh
AU - Takeoka, Shinji
AU - Kiyosawa, Tomoharu
AU - Kinoshita, Manabu
N1 - Funding Information:
Source of Funding: This work was supported in part by a grant-in-aid for Scientific Research (B) (No. 25289252) (S.T.) from JSPS; Mizuho Foundation for the Promotion of Sciences (T.F.); a grant-in-aid for the Special Research Program from the National Defense Medical College (D.S.); a grant-in-aid for Advanced Research on Military Medicine from Ministry of Defense (M.K., T.K.).
Publisher Copyright:
© 2018 by the Wound Healing Society
PY - 2017/11/1
Y1 - 2017/11/1
N2 - Although human recombinant basic fibroblast growth factor (bFGF) is widely used for wound healing, daily treatment with bFGF is required because of its short half-life. An effective controlled-release system of bFGF is, therefore, desired in clinical settings. To investigate the efficacy of a bFGF-loaded nanosheet for wound healing, focusing on the controlled-release of bFGF, bFGF-loaded poly(lactic-co-glycolic acid) (PGLA) nanosheets were developed, and their in vitro release profile of bFGF and their in vivo efficacy for wound healing were examined. A polyion complex of positively charged human recombinant bFGF and negatively charged alginate was sandwiched between PLGA nanosheets (70 nm thick for each layer). The resulting bFGF-loaded nanosheet robustly adhered to silicon skin by observation using a microscratch test. bFGF was gradually and continuously released over three days in an in vitro incubation study. Treatment with the bFGF-loaded nanosheets (every 3 day for 15 days) as well as with a conventional bFGF spray effectively promoted wound healing of mouse dorsal skin defects with accelerated tissue granulation and angiogenesis, although the dose of bFGF used in the treatment with the bFGF nanosheets was approximately 1/20 of the sprayed bFGF. In conclusion, we developed a bFGF-loaded nanosheet that sustained a continuous release of bFGF over three days and effectively promoted wound healing in mice.
AB - Although human recombinant basic fibroblast growth factor (bFGF) is widely used for wound healing, daily treatment with bFGF is required because of its short half-life. An effective controlled-release system of bFGF is, therefore, desired in clinical settings. To investigate the efficacy of a bFGF-loaded nanosheet for wound healing, focusing on the controlled-release of bFGF, bFGF-loaded poly(lactic-co-glycolic acid) (PGLA) nanosheets were developed, and their in vitro release profile of bFGF and their in vivo efficacy for wound healing were examined. A polyion complex of positively charged human recombinant bFGF and negatively charged alginate was sandwiched between PLGA nanosheets (70 nm thick for each layer). The resulting bFGF-loaded nanosheet robustly adhered to silicon skin by observation using a microscratch test. bFGF was gradually and continuously released over three days in an in vitro incubation study. Treatment with the bFGF-loaded nanosheets (every 3 day for 15 days) as well as with a conventional bFGF spray effectively promoted wound healing of mouse dorsal skin defects with accelerated tissue granulation and angiogenesis, although the dose of bFGF used in the treatment with the bFGF nanosheets was approximately 1/20 of the sprayed bFGF. In conclusion, we developed a bFGF-loaded nanosheet that sustained a continuous release of bFGF over three days and effectively promoted wound healing in mice.
UR - http://www.scopus.com/inward/record.url?scp=85041720848&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85041720848&partnerID=8YFLogxK
U2 - 10.1111/wrr.12604
DO - 10.1111/wrr.12604
M3 - Article
C2 - 29315978
AN - SCOPUS:85041720848
SN - 1067-1927
VL - 25
SP - 1008
EP - 1016
JO - Wound Repair and Regeneration
JF - Wound Repair and Regeneration
IS - 6
ER -