The main factor of the decrease in activity of luciferase on the Si surface

Katsuhiko Nishiyama; Takanobu Watanabe; Tadatsugu Hoshino; Iwao Ohdomari

doi:10.1016/j.cplett.2008.01.040

The main factor of the decrease in activity of luciferase on the Si surface

Katsuhiko Nishiyama^*, Takanobu Watanabe, Tadatsugu Hoshino, Iwao Ohdomari

^*Corresponding author for this work

School of Fundamental Science and Engineering

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The interactions between the luciferase surface area around the active site and the Si surface were estimated by molecular dynamics simulations. The results show that the luciferase surface area around the active site is more unstable than the luciferase surface area with relatively high hydrophobicity when luciferase adsorbs on the Si surface. The main factor of the decrease in activity of luciferase on the Si surface would be adsorption-induced structural changes in active site. Si surface should be designed focusing on structural changes in active site.

Original language	English
Pages (from-to)	279-282
Number of pages	4
Journal	Chemical Physics Letters
Volume	453
Issue number	4-6
DOIs	https://doi.org/10.1016/j.cplett.2008.01.040
Publication status	Published - 2008 Mar 3

ASJC Scopus subject areas

Physics and Astronomy(all)
Physical and Theoretical Chemistry

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10.1016/j.cplett.2008.01.040

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title = "The main factor of the decrease in activity of luciferase on the Si surface",

abstract = "The interactions between the luciferase surface area around the active site and the Si surface were estimated by molecular dynamics simulations. The results show that the luciferase surface area around the active site is more unstable than the luciferase surface area with relatively high hydrophobicity when luciferase adsorbs on the Si surface. The main factor of the decrease in activity of luciferase on the Si surface would be adsorption-induced structural changes in active site. Si surface should be designed focusing on structural changes in active site.",

author = "Katsuhiko Nishiyama and Takanobu Watanabe and Tadatsugu Hoshino and Iwao Ohdomari",

note = "Funding Information: This study is supported by a Grant-in-Aid for Center of Excellence (COE) Research from the Ministry of Education, Culture, Sports, Science and Technology, by a Grant for the Promotion of the Advancement of Education and Research in Graduate Schools from the Promotion and Mutual Aid Corporation for Private Schools of Japan, and by JST-PRESTO.",

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T1 - The main factor of the decrease in activity of luciferase on the Si surface

AU - Nishiyama, Katsuhiko

AU - Watanabe, Takanobu

AU - Hoshino, Tadatsugu

AU - Ohdomari, Iwao

N1 - Funding Information: This study is supported by a Grant-in-Aid for Center of Excellence (COE) Research from the Ministry of Education, Culture, Sports, Science and Technology, by a Grant for the Promotion of the Advancement of Education and Research in Graduate Schools from the Promotion and Mutual Aid Corporation for Private Schools of Japan, and by JST-PRESTO.

PY - 2008/3/3

Y1 - 2008/3/3

N2 - The interactions between the luciferase surface area around the active site and the Si surface were estimated by molecular dynamics simulations. The results show that the luciferase surface area around the active site is more unstable than the luciferase surface area with relatively high hydrophobicity when luciferase adsorbs on the Si surface. The main factor of the decrease in activity of luciferase on the Si surface would be adsorption-induced structural changes in active site. Si surface should be designed focusing on structural changes in active site.

AB - The interactions between the luciferase surface area around the active site and the Si surface were estimated by molecular dynamics simulations. The results show that the luciferase surface area around the active site is more unstable than the luciferase surface area with relatively high hydrophobicity when luciferase adsorbs on the Si surface. The main factor of the decrease in activity of luciferase on the Si surface would be adsorption-induced structural changes in active site. Si surface should be designed focusing on structural changes in active site.

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JF - Chemical Physics Letters

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The main factor of the decrease in activity of luciferase on the Si surface

Abstract

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