The natural intron sequence of human tyrosine pre-transfer RNA is not a temporal stabilizer for cloverleaf structure

Tomoaki Ando; Terumichi Tanaka; Yo Kikuchi

doi:10.1271/bbb.68.1782

The natural intron sequence of human tyrosine pre-transfer RNA is not a temporal stabilizer for cloverleaf structure

Tomoaki Ando, Terumichi Tanaka^*, Yo Kikuchi

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

We have developed the hyperprocessing technique to evaluate the stability of the cloverleaf shape of pretransfer RNA (pre-tRNA). Application of this strategy to hyperprocessible human tyrosine pre-tRNA indicated that the natural intron sequence did not contribute to stabilization of the cloverleaf shape of this pre-tRNA, while the artificial intron with elongated anticodon-stem completely inhibited hyperprocessing of it. Our data suggested that the contemporary intron sequence may be a vestige of the ancient pre-biotic world, but not has been retained as a temporal stabilizer of the pre-tRNA before the base modifications.

Original language	English
Pages (from-to)	1782-1785
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	68
Issue number	8
DOIs	https://doi.org/10.1271/bbb.68.1782
Publication status	Published - 2004 Aug
Externally published	Yes

Keywords

Escherichia coli
Hyperprocessing
Ribonuclease P
Tyrosine transfer RNA (tRNA)

ASJC Scopus subject areas

Food Science
Applied Microbiology and Biotechnology
Chemistry (miscellaneous)
Biochemistry, Genetics and Molecular Biology(all)
Biochemistry
Biotechnology

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abstract = "We have developed the hyperprocessing technique to evaluate the stability of the cloverleaf shape of pretransfer RNA (pre-tRNA). Application of this strategy to hyperprocessible human tyrosine pre-tRNA indicated that the natural intron sequence did not contribute to stabilization of the cloverleaf shape of this pre-tRNA, while the artificial intron with elongated anticodon-stem completely inhibited hyperprocessing of it. Our data suggested that the contemporary intron sequence may be a vestige of the ancient pre-biotic world, but not has been retained as a temporal stabilizer of the pre-tRNA before the base modifications.",

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T1 - The natural intron sequence of human tyrosine pre-transfer RNA is not a temporal stabilizer for cloverleaf structure

AU - Ando, Tomoaki

AU - Tanaka, Terumichi

AU - Kikuchi, Yo

PY - 2004/8

Y1 - 2004/8

N2 - We have developed the hyperprocessing technique to evaluate the stability of the cloverleaf shape of pretransfer RNA (pre-tRNA). Application of this strategy to hyperprocessible human tyrosine pre-tRNA indicated that the natural intron sequence did not contribute to stabilization of the cloverleaf shape of this pre-tRNA, while the artificial intron with elongated anticodon-stem completely inhibited hyperprocessing of it. Our data suggested that the contemporary intron sequence may be a vestige of the ancient pre-biotic world, but not has been retained as a temporal stabilizer of the pre-tRNA before the base modifications.

AB - We have developed the hyperprocessing technique to evaluate the stability of the cloverleaf shape of pretransfer RNA (pre-tRNA). Application of this strategy to hyperprocessible human tyrosine pre-tRNA indicated that the natural intron sequence did not contribute to stabilization of the cloverleaf shape of this pre-tRNA, while the artificial intron with elongated anticodon-stem completely inhibited hyperprocessing of it. Our data suggested that the contemporary intron sequence may be a vestige of the ancient pre-biotic world, but not has been retained as a temporal stabilizer of the pre-tRNA before the base modifications.

KW - Escherichia coli

KW - Hyperprocessing

KW - Ribonuclease P

KW - Tyrosine transfer RNA (tRNA)

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JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 8

ER -

The natural intron sequence of human tyrosine pre-transfer RNA is not a temporal stabilizer for cloverleaf structure

Abstract

Keywords

ASJC Scopus subject areas

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