The PURE system for protein production

Yoshihiro Shimizu, Yutetsu Kuruma, Takashi Kanamori, Takuya Ueda

Research output: Chapter in Book/Report/Conference proceedingChapter

28 Citations (Scopus)


In the field of molecular biology or biochemistry, preparation and use of purified proteins involved in a certain biological system is crucial for understanding their mechanisms and functions in cells or organisms. The recent progress in a cell-free translation system allows us to prepare proteins in a test tube directly from cDNAs that encode the amino acid sequences. The use of the reconstituted cell-free translation system termed PURE (Protein synthesis Using Recombinant Elements) for these purposes is effective in several applications. Here we describe methods of recombinant protein expression using the PURE system for molecular biological or biochemical studies.

Original languageEnglish
Title of host publicationCell-Free Protein Synthesis
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages10
ISBN (Print)9781627037815
Publication statusPublished - 2014
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Cell-free protein synthesis system
  • In vitro translation system
  • PURE system
  • Ribosome
  • tRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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