The rapid quantification and detection of nitrifying bacteria by using monoclonal antibody method

H. Ikuta; N. Noda; Y. Ebie; A. Hirata; S. Tsuneda; M. Matsumura; Y. Inamori

doi:10.2166/wst.2000.0351

The rapid quantification and detection of nitrifying bacteria by using monoclonal antibody method

H. Ikuta^*, N. Noda, Y. Ebie, A. Hirata, S. Tsuneda, M. Matsumura, Y. Inamori

^*Corresponding author for this work

Research output: Contribution to journal › Conference article › peer-review

4 Citations (Scopus)

Abstract

Monoclonal antibodies against the two kinds of nitrifying bacteria Nitrosomonas europaea (IFO 14298) and Nitrobacter winogradskyi (IFO 14297) were raised and isotypes of these monoclonal antibodies, IgM and IgG₁, were successfully obtained. Cross reactivities of these monoclonal antibodies against various kinds of representative heterotrophic bacteria turned out to be relatively low by competitive ELISA. In contrast, these monoclonal antibodies were very specific for nitrifying bacteria used as antigens. By means of sandwich ELISA using different isotype monoclonal antibodies such as IgM and IgG₁, calibration curves were successfully developed for quantification of nitrifying bacteria. It was shown that the obtainable lower limit of quantification of N. europaea and N. winogradskyi were 7.0 × 10⁶ N/ml and were 6.0 × 10⁵ N/ml, respectively. Nitrifying bacteria in activated sludge of advanced domestic wastewater treatment johkaso were counted by sandwich ELISA and MPN methods. The bacterial number estimated by MPN method was lower than that estimated by sandwich ELISA. It was indicated that this monoclonal antibody method could be used as a quick and powerful tool for estimating and controlling the population of nitrifying bacteria in the advanced domestic wastewater treatment processes.

Original language	English
Pages (from-to)	1-7
Number of pages	7
Journal	Water Science and Technology
Volume	42
Issue number	3-4
DOIs	https://doi.org/10.2166/wst.2000.0351
Publication status	Published - 2000
Externally published	Yes
Event	Water Quality Management in Asia (Asian Waterqual'99) - Taipei, Taiwan Duration: 1999 Oct 18 → 1999 Oct 20

Keywords

Monoclonal antibodies
Nitrifying bacteria
Quantification
Sandwich ELISA

ASJC Scopus subject areas

Environmental Engineering
Water Science and Technology

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10.2166/wst.2000.0351

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title = "The rapid quantification and detection of nitrifying bacteria by using monoclonal antibody method",

abstract = "Monoclonal antibodies against the two kinds of nitrifying bacteria Nitrosomonas europaea (IFO 14298) and Nitrobacter winogradskyi (IFO 14297) were raised and isotypes of these monoclonal antibodies, IgM and IgG1, were successfully obtained. Cross reactivities of these monoclonal antibodies against various kinds of representative heterotrophic bacteria turned out to be relatively low by competitive ELISA. In contrast, these monoclonal antibodies were very specific for nitrifying bacteria used as antigens. By means of sandwich ELISA using different isotype monoclonal antibodies such as IgM and IgG1, calibration curves were successfully developed for quantification of nitrifying bacteria. It was shown that the obtainable lower limit of quantification of N. europaea and N. winogradskyi were 7.0 × 106 N/ml and were 6.0 × 105 N/ml, respectively. Nitrifying bacteria in activated sludge of advanced domestic wastewater treatment johkaso were counted by sandwich ELISA and MPN methods. The bacterial number estimated by MPN method was lower than that estimated by sandwich ELISA. It was indicated that this monoclonal antibody method could be used as a quick and powerful tool for estimating and controlling the population of nitrifying bacteria in the advanced domestic wastewater treatment processes.",

keywords = "Monoclonal antibodies, Nitrifying bacteria, Quantification, Sandwich ELISA",

author = "H. Ikuta and N. Noda and Y. Ebie and A. Hirata and S. Tsuneda and M. Matsumura and Y. Inamori",

year = "2000",

doi = "10.2166/wst.2000.0351",

language = "English",

volume = "42",

pages = "1--7",

journal = "Water Science and Technology",

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TY - JOUR

T1 - The rapid quantification and detection of nitrifying bacteria by using monoclonal antibody method

AU - Ikuta, H.

AU - Noda, N.

AU - Ebie, Y.

AU - Hirata, A.

AU - Tsuneda, S.

AU - Matsumura, M.

AU - Inamori, Y.

PY - 2000

Y1 - 2000

N2 - Monoclonal antibodies against the two kinds of nitrifying bacteria Nitrosomonas europaea (IFO 14298) and Nitrobacter winogradskyi (IFO 14297) were raised and isotypes of these monoclonal antibodies, IgM and IgG1, were successfully obtained. Cross reactivities of these monoclonal antibodies against various kinds of representative heterotrophic bacteria turned out to be relatively low by competitive ELISA. In contrast, these monoclonal antibodies were very specific for nitrifying bacteria used as antigens. By means of sandwich ELISA using different isotype monoclonal antibodies such as IgM and IgG1, calibration curves were successfully developed for quantification of nitrifying bacteria. It was shown that the obtainable lower limit of quantification of N. europaea and N. winogradskyi were 7.0 × 106 N/ml and were 6.0 × 105 N/ml, respectively. Nitrifying bacteria in activated sludge of advanced domestic wastewater treatment johkaso were counted by sandwich ELISA and MPN methods. The bacterial number estimated by MPN method was lower than that estimated by sandwich ELISA. It was indicated that this monoclonal antibody method could be used as a quick and powerful tool for estimating and controlling the population of nitrifying bacteria in the advanced domestic wastewater treatment processes.

AB - Monoclonal antibodies against the two kinds of nitrifying bacteria Nitrosomonas europaea (IFO 14298) and Nitrobacter winogradskyi (IFO 14297) were raised and isotypes of these monoclonal antibodies, IgM and IgG1, were successfully obtained. Cross reactivities of these monoclonal antibodies against various kinds of representative heterotrophic bacteria turned out to be relatively low by competitive ELISA. In contrast, these monoclonal antibodies were very specific for nitrifying bacteria used as antigens. By means of sandwich ELISA using different isotype monoclonal antibodies such as IgM and IgG1, calibration curves were successfully developed for quantification of nitrifying bacteria. It was shown that the obtainable lower limit of quantification of N. europaea and N. winogradskyi were 7.0 × 106 N/ml and were 6.0 × 105 N/ml, respectively. Nitrifying bacteria in activated sludge of advanced domestic wastewater treatment johkaso were counted by sandwich ELISA and MPN methods. The bacterial number estimated by MPN method was lower than that estimated by sandwich ELISA. It was indicated that this monoclonal antibody method could be used as a quick and powerful tool for estimating and controlling the population of nitrifying bacteria in the advanced domestic wastewater treatment processes.

KW - Monoclonal antibodies

KW - Nitrifying bacteria

KW - Quantification

KW - Sandwich ELISA

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T2 - Water Quality Management in Asia (Asian Waterqual'99)

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The rapid quantification and detection of nitrifying bacteria by using monoclonal antibody method

Abstract

Keywords

ASJC Scopus subject areas

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