The role of GABAergic neuron on NMDA- and SP-induced phase delays in the suprachiasmatic nucleus neuronal activity rhythm in vitro

Toshiyuki Hamada; Shigenobu Shibata

doi:10.1016/j.neulet.2009.11.029

The role of GABAergic neuron on NMDA- and SP-induced phase delays in the suprachiasmatic nucleus neuronal activity rhythm in vitro

Toshiyuki Hamada, Shigenobu Shibata^*

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Gamma-aminobutyric acid (GABA), and its biosynthetic enzyme, glutamic decarboxylase, are widely distributed in the suprachiasmatic nucleus (SCN). In the present study, we examined the role of the GABA_A receptor on in vitro SCN responses to photic-like signals. We found that 100 μM GABA_A receptor antagonist bicuculline partially blocked field potentials evoked by optic nerve stimulation. NMDA- and SP-induced phase shifts of SCN neuronal activity rhythms, were blocked with 10 μM bicuculline. Application of 100 μM bicuculline alone induced phase advance of SCN neuronal activity rhythm. These results show that NMDA- and SP-induced phase shifts are blocked by bicuculline and suggest GABA has an important role as neurotransmitter in the neuronal network regulating phase shifts of the circadian clock.

Original language	English
Pages (from-to)	344-347
Number of pages	4
Journal	Neuroscience Letters
Volume	468
Issue number	3
DOIs	https://doi.org/10.1016/j.neulet.2009.11.029
Publication status	Published - 2010 Jan 14

Keywords

Bicuculline
Circadian rhythm
Field potential
GABA
SCN firing rhythm
Suprachiasmatic nucleus

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/j.neulet.2009.11.029

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title = "The role of GABAergic neuron on NMDA- and SP-induced phase delays in the suprachiasmatic nucleus neuronal activity rhythm in vitro",

abstract = "Gamma-aminobutyric acid (GABA), and its biosynthetic enzyme, glutamic decarboxylase, are widely distributed in the suprachiasmatic nucleus (SCN). In the present study, we examined the role of the GABAA receptor on in vitro SCN responses to photic-like signals. We found that 100 μM GABAA receptor antagonist bicuculline partially blocked field potentials evoked by optic nerve stimulation. NMDA- and SP-induced phase shifts of SCN neuronal activity rhythms, were blocked with 10 μM bicuculline. Application of 100 μM bicuculline alone induced phase advance of SCN neuronal activity rhythm. These results show that NMDA- and SP-induced phase shifts are blocked by bicuculline and suggest GABA has an important role as neurotransmitter in the neuronal network regulating phase shifts of the circadian clock.",

keywords = "Bicuculline, Circadian rhythm, Field potential, GABA, SCN firing rhythm, Suprachiasmatic nucleus",

author = "Toshiyuki Hamada and Shigenobu Shibata",

note = "Funding Information: We thank Dr. Mike Antle for comments on drafts of this manuscript. This study was supported by Grant-in-Aid for Young Scientists (B) ( 15790127 ) from MEXT (to T.H.).",

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AU - Hamada, Toshiyuki

AU - Shibata, Shigenobu

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N2 - Gamma-aminobutyric acid (GABA), and its biosynthetic enzyme, glutamic decarboxylase, are widely distributed in the suprachiasmatic nucleus (SCN). In the present study, we examined the role of the GABAA receptor on in vitro SCN responses to photic-like signals. We found that 100 μM GABAA receptor antagonist bicuculline partially blocked field potentials evoked by optic nerve stimulation. NMDA- and SP-induced phase shifts of SCN neuronal activity rhythms, were blocked with 10 μM bicuculline. Application of 100 μM bicuculline alone induced phase advance of SCN neuronal activity rhythm. These results show that NMDA- and SP-induced phase shifts are blocked by bicuculline and suggest GABA has an important role as neurotransmitter in the neuronal network regulating phase shifts of the circadian clock.

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KW - Bicuculline

KW - Circadian rhythm

KW - Field potential

KW - GABA

KW - SCN firing rhythm

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The role of GABAergic neuron on NMDA- and SP-induced phase delays in the suprachiasmatic nucleus neuronal activity rhythm in vitro

Abstract

Keywords

ASJC Scopus subject areas

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